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一种可注射的髓核细胞修饰的脱细胞支架：用于预防椎间盘退变的生物相容性材料。

An injectable nucleus pulposus cell-modified decellularized scaffold: biocompatible material for prevention of disc degeneration.

作者信息

Shan Zhi, Lin Xianfeng, Wang Shengyu, Zhang Xuyang, Pang Yichuan, Li Shengyun, Yu Tianming, Fan Shunwu, Zhao Fengdong

机构信息

Department of Orthopaedic Surgery, Sir Run Run Shaw Hospital, Medical College of Zhejiang University, Hangzhou, 310016, China.

MOE Key Laboratory of Macromolecular synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou, 310027, China.

出版信息

Oncotarget. 2017 Jun 20;8(25):40276-40288. doi: 10.18632/oncotarget.16831.

DOI:10.18632/oncotarget.16831
PMID:28402966
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5522320/
Abstract

We developed a nucleus pulposus cell (NPC)-modulated decellularized small intestinal submucosa (SIS) scaffold, and assessed the ability of this material to prevent Intervertebral disc degeneration (IVD) degeneration. Decellularized porcine SIS was squashed into particles and the biological safety and efficiency of these particles were evaluated. Next, SIS particles were seeded with rabbit NPCs, cultured for two months in vitro, decellularized again and suspended for intervertebral injection. We demonstrated that use of the decellularization protocol resulted in the removal of cellular components with maximal retention of extracellular matrix. The xenogeneic decellularized SIS did not display cytotoxicity in vitro and its application prevented NPC degradation. Furthermore, the xenogeneic SIS microparticles were effective in preventing IVD progression in vivo in a rabbit disc degeneration model. In conclusion, our study describes an optimized method for decellularized SIS preparation and demonstrated that the material is safe and effective for treating IVD degeneration.

摘要

我们研发了一种由髓核细胞（NPC）调控的去细胞小肠黏膜下层（SIS）支架，并评估了该材料预防椎间盘退变（IVD）的能力。将去细胞猪SIS挤压成颗粒，并对这些颗粒的生物安全性和有效性进行评估。接下来，将兔NPC接种到SIS颗粒上，在体外培养两个月，再次去细胞并悬浮用于椎间注射。我们证明，使用去细胞方案可去除细胞成分，同时最大程度保留细胞外基质。异种去细胞SIS在体外未显示出细胞毒性，其应用可防止NPC降解。此外，异种SIS微粒在兔椎间盘退变模型中可有效预防体内IVD进展。总之，我们的研究描述了一种优化的去细胞SIS制备方法，并证明该材料在治疗IVD退变方面安全有效。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab00/5522320/1a32697ffe13/oncotarget-08-40276-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab00/5522320/985dcf6af02c/oncotarget-08-40276-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab00/5522320/1625b44392d0/oncotarget-08-40276-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab00/5522320/0bbe429ed18f/oncotarget-08-40276-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab00/5522320/fd613d86e7ea/oncotarget-08-40276-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab00/5522320/a81032c9fc0f/oncotarget-08-40276-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab00/5522320/f7dd784c2d0a/oncotarget-08-40276-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab00/5522320/1a32697ffe13/oncotarget-08-40276-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab00/5522320/985dcf6af02c/oncotarget-08-40276-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab00/5522320/1625b44392d0/oncotarget-08-40276-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab00/5522320/0bbe429ed18f/oncotarget-08-40276-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab00/5522320/fd613d86e7ea/oncotarget-08-40276-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab00/5522320/a81032c9fc0f/oncotarget-08-40276-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab00/5522320/f7dd784c2d0a/oncotarget-08-40276-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab00/5522320/1a32697ffe13/oncotarget-08-40276-g007.jpg

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本文引用的文献

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2
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Theor Biol Med Model. 2015 Nov 9;12:24. doi: 10.1186/s12976-015-0020-3.
3
An understanding of intervertebral disc development, maturation and cell phenotype provides clues to direct cell-based tissue regeneration therapies for disc degeneration.
用于修复椎间盘退变的脱细胞基质：制备方法、应用及动物模型
Mater Today Bio. 2022 Dec 17;18:100523. doi: 10.1016/j.mtbio.2022.100523. eCollection 2023 Feb.
4
The application of decellularized nucleus pulposus matrix/chitosan with transforming growth factor β3 for nucleus pulposus tissue engineering.脱细胞髓核基质/壳聚糖与转化生长因子β3在髓核组织工程中的应用
Cytotechnology. 2021 Jun;73(3):447-456. doi: 10.1007/s10616-021-00469-0. Epub 2021 Apr 15.
5
The role of lncRNA MALAT1 in intervertebral degenerative disc disease.长链非编码RNA MALAT1在椎间盘退变疾病中的作用。
Int J Clin Exp Pathol. 2017 Oct 1;10(10):10611-10617. eCollection 2017.
6
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J Biomed Mater Res B Appl Biomater. 2019 Nov;107(8):2488-2499. doi: 10.1002/jbm.b.34339. Epub 2019 Feb 15.
对椎间盘发育、成熟及细胞表型的了解为指导基于细胞的椎间盘退变组织再生疗法提供了线索。
Eur Spine J. 2014 Sep;23(9):1803-14. doi: 10.1007/s00586-014-3305-z. Epub 2014 Apr 29.
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6
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