Bepler G, Koehler A, Kiefer P, Havemann K, Beisenherz K, Jaques G, Gropp C, Haeder M
Philipps University Medical Center, Department of Internal Medicine, Marburg, Federal Republic of Germany.
Differentiation. 1988;37(2):158-71. doi: 10.1111/j.1432-0436.1988.tb00806.x.
Six new non-small-cell lung cancer (NSCLC) cell lines were established directly from human tissue or indirectly via nude mouse xenografts in serum-supplemented media with success rates of 8% and 13%, respectively. They comprised one adenocarcinoma (ADLC-5M2), two squamous cell carcinomas (EPLC-32M1, EPLC-65H), two large cell carcinomas (LCLC-97TM1, LCLC-103H), and one malignant biphasic mesothelioma (MSTO-211H). All cell lines grew adherent to culture vessels with population doubling times (PDT) of 16-40 h, formed colonies in soft agarose with efficiencies of 0.1%-5.1%, and all grew in athymic nude mice. Xenograft histologies appeared as follows: (a) undifferentiated carcinomas with feeble resemblance to the original tumors in the case of adenocarcinomas and squamous cell carcinomas; (b) large cell carcinoma with high resemblance to the original tumor; (c) an undifferentiated tumor with predominance of large epithelial cells and few fibrous cells in the case of mesothelioma. Human chorionic gonadotropin (HCG) was found by radioimmunoassay and high-affinity binding sites for epidermal growth factor (EGF) by radio-receptor assay in 4/4 cell lines. A very low activity of L-DOPA decarboxylase (DDC) was detectable only in the adenocarcinoma cell line. All cell lines overexpressed the c-myc protooncogene, and no gene rearrangement or amplification was observed. Chromosome analysis revealed modal chromosome numbers of 70-73 in ADLC-5M2, EPLC-32M1, EPLC-65H, and MSTO-211H. Cell lines derived from large cell carcinoma had modal values of 65 and 170 and a wider chromosome distribution than all other cell lines. A NSCLC specific chromosomal aberration has been undetectable until now. These cell lines may aid in elucidating the biology of NSCLC and its interrelationship to other lung tumors.
六种新的非小细胞肺癌（NSCLC）细胞系分别直接从人体组织或通过裸鼠异种移植间接在补充血清的培养基中建立，成功率分别为8%和13%。它们包括1例腺癌（ADLC - 5M2）、2例鳞状细胞癌（EPLC - 32M1、EPLC - 65H）、2例大细胞癌（LCLC - 97TM1、LCLC - 103H）和1例恶性双相性间皮瘤（MSTO - 211H）。所有细胞系均贴壁生长于培养容器，群体倍增时间（PDT）为16 - 40小时，在软琼脂糖中形成集落的效率为0.1% - 5.1%，且均能在无胸腺裸鼠体内生长。异种移植组织学表现如下：（a）腺癌和鳞状细胞癌的未分化癌与原发肿瘤相似性较弱；（b）大细胞癌与原发肿瘤高度相似；（c）间皮瘤的未分化肿瘤以大上皮细胞为主，纤维细胞较少。通过放射免疫测定在4/4细胞系中检测到人类绒毛膜促性腺激素（HCG），通过放射受体测定在4/4细胞系中检测到表皮生长因子（EGF）的高亲和力结合位点。仅在腺癌细胞系中可检测到极低活性的L - 多巴脱羧酶（DDC）。所有细胞系均过度表达c - myc原癌基因，未观察到基因重排或扩增。染色体分析显示，ADLC - 5M2、EPLC - 32M1、EPLC - 65H和MSTO - 211H的众数染色体数为70 - 73。源自大细胞癌的细胞系众数为65和170，且染色体分布比所有其他细胞系更广泛。迄今为止，尚未检测到NSCLC特异性染色体畸变。这些细胞系可能有助于阐明NSCLC的生物学特性及其与其他肺肿瘤的相互关系。
