Takata H, Yoshino T, Hoshida Y, Takata I, Akagi T
Department of Pathology, Okayama University Medical School, Japan.
Acta Med Okayama. 1992 Aug;46(4):257-64. doi: 10.18926/AMO/32620.
A cell line of human lung large cell carcinoma (LCC) was established directly from the metastatic skin tumor tissue. The clinical course of the patient who carried this carcinoma was peculiar; generalized lymphadenopathy, histologically resembling Hodgkin's disease, was found as the first clinical symptom. The lung tumor was not discovered until the time of autopsy. This cell line (KaMi) grew adherent to culture vessels with the population doubling time of 20.6h, formed colonies in soft agars with efficiency of 22.6%, and formed tumors in athymic nude mice. The authenticity of KaMi was confirmed by chromosomal analysis and isoenzyme patterns. KaMi cells bore a strong resemblance to the original tumor cells which were composed of small spindle cells, large polygonal cells, and multinucleated giant cells. Immunohistochemically, KaMi cells showed a weak tendency to differentiate to squamous cells, and these immunohistochemical reactivities were almost compatible to those of the original tumor cells, but ultrastructurally, KaMi cells were more immature than the original ones. Treatment with several reagents could not augment a differentiation of KaMi cells. Cytokeratin profiles showed a tendency of squamous cell differentiation. KaMi cells may aid in elucidating the pathogenesis and biology of LCC and its relationship to other lung tumors.
一株人肺大细胞癌（LCC）细胞系直接从转移性皮肤肿瘤组织中建立。携带这种癌症的患者临床病程独特；作为首个临床症状，发现有组织学上类似于霍奇金病的全身淋巴结病。直到尸检时才发现肺部肿瘤。该细胞系（KaMi）贴壁生长于培养容器，群体倍增时间为20.6小时，在软琼脂中形成集落的效率为22.6%，并在无胸腺裸鼠体内形成肿瘤。通过染色体分析和同工酶谱证实了KaMi的真实性。KaMi细胞与由小梭形细胞、大的多角形细胞和多核巨细胞组成的原始肿瘤细胞极为相似。免疫组织化学分析显示，KaMi细胞有向鳞状细胞分化的微弱倾向，这些免疫组织化学反应与原始肿瘤细胞的反应几乎一致，但在超微结构上，KaMi细胞比原始细胞更不成熟。用几种试剂处理不能增强KaMi细胞的分化。细胞角蛋白谱显示有鳞状细胞分化的倾向。KaMi细胞可能有助于阐明LCC的发病机制和生物学特性及其与其他肺部肿瘤的关系。
