Universidade Estadual de São Paulo - UNESP, School of Dentistry of Araraquara, Department of Diagnosis and Surgery, Araraquara, SP, Brazil.
Centro Universitário Unievangélica, Department of Periodontology, Anápolis, GO, Brazil.
Braz Oral Res. 2017 Apr 10;31:e28. doi: 10.1590/1807-3107BOR-2017.vol31.0028.
The purpose of this study is to evaluate the effect of the avocado/soybean unsaponifiables (ASU) on the treatment of induced periodontitis in rats. Periodontitis was induced in 84 rats via ligature placement around the second upper molar, which was removed after 7 days, and scaling and root planning (SRP) was performed at this time. Subsequently, the rats were randomly allocated to four groups with 21 animals each: One SRP group in which saline solution was administered (SS), and three groups in which ASU was administered (0.6 g/kg/day), beginning either 7 days before the induction of periodontitis (SRP/ASU-7), on the day of periodontitis induction (SRP/ASU0), or on the day of treatment (SRP/ASU+7). ASU and SS were administered daily by gavage until the sacrifice of the animals (7, 15, and 30 days after SRP). The % bone in the furcation area was evaluated by histomorphometry and micro-CT. The expression of proteins (TRAP, RANKL, and alkaline phosphatase) and mRNA (IL-1β, TNF-α, IL-6, RANKL, and alkaline phosphatase) were evaluated by immunohistochemistry and qPCR. The SRP/ASU+7 group presented a higher percentage of bone fill in the furcation area and higher expression of alkaline phosphatase than in the SRP group (at 7 and 30 days, respectively). The SRP/ASU0 and SRP/ASU+7 groups presented lower expression levels of RANKL mRNA than the SRP and SRP/ASU-7 groups at 15 days. ASU administration on the day of the SRP treatment of the ligature-induced periodontitis promoted subtle beneficial effects on periodontal repair following the treatment of induced periodontitis within the experimental period of 7 days.
本研究旨在评估鳄梨/大豆不皂化物（ASU）对诱导性大鼠牙周炎治疗的影响。通过在上颌第二磨牙周围放置结扎线来诱导牙周炎，7 天后去除结扎线，并在此时进行牙周洁治和根面平整术（SRP）。随后，将大鼠随机分为 4 组，每组 21 只：一组给予生理盐水（SS），三组给予 ASU（0.6 g/kg/天），分别在牙周炎诱导前 7 天（SRP/ASU-7）、牙周炎诱导当天（SRP/ASU0）或治疗当天（SRP/ASU+7）开始给药。通过灌胃每日给予 ASU 和 SS，直至处死动物（SRP 后 7、15 和 30 天）。通过组织形态计量学和微 CT 评估分叉区的骨百分比。通过免疫组织化学和 qPCR 评估蛋白质（TRAP、RANKL 和碱性磷酸酶）和 mRNA（IL-1β、TNF-α、IL-6、RANKL 和碱性磷酸酶）的表达。与 SRP 组相比，SRP/ASU+7 组在第 7 天和第 30 天的分叉区骨填充百分比和碱性磷酸酶表达更高。SRP/ASU0 组和 SRP/ASU+7 组在第 15 天的 RANKL mRNA 表达水平低于 SRP 组和 SRP/ASU-7 组。在结扎诱导的牙周炎的 SRP 治疗当天给予 ASU 治疗，在 7 天的实验期间对诱导性牙周炎的治疗后牙周修复有轻微的有益作用。
