de Paula Luiz Guilherme Freitas, Lopes de Oliveira Guilherme José Pimentel, Pinotti Felipe Eduardo, Grecchi Bruna Biagioni, de Aquino Sabrina Garcia, Chiérici Marcantonio Rosemary Adriana
Int J Oral Maxillofac Implants. 2018 May/Jun;33(3):603-612. doi: 10.11607/jomi.6124.
To evaluate the influence of the use of avocado/soybean unsaponifiables (ASU) on osseointegration of implants in animals with experimental arthritis.
One hundred twenty rats were randomly divided into four groups: CTR, healthy animals and saline solution administration; ASU, healthy animals and ASU administration; ART, arthritic animals and saline solution administration; and ART/ASU, arthritic animals and ASU administration. The solutions were administered daily by gavage, beginning 7 days before the surgical procedures until the completion of the experimental period (15, 30, and 60 days after the placement of the implants in the tibia). The osseointegration of the implants was evaluated by histometric analysis (bone-to-implant contact [% BIC], bone area between the threads [% BBT]) and biomechanical analysis. Microcomputed tomography (micro-CT) analysis was used to assess bone volume in the vicinity of the implant. Immunohistochemistry analysis was performed to assess the expression of osteocalcin and transforming growth factor beta 1 (TGF-β1).
The ART/ASU group showed a decreased percentage of bone in the area around the implant compared with the ASU and ART groups (15 and 30 days). The ART/ASU group showed increased removal torque values (30 days) and % BIC and % BBT (30 to 60 days) compared with the ART group. The ASU group had increased % BIC values compared with the ART and CTR groups (60 days). The CTR group had the highest expression of osteocalcin, while the ASU group presented the highest expression of TGF-β1 at 60 days.
The ASU administration improved the osseointegration, particularly in animals with induced arthritis.
评估使用鳄梨/大豆不皂化物（ASU）对实验性关节炎动物种植体骨整合的影响。
120只大鼠随机分为四组：CTR组，健康动物并给予生理盐水；ASU组，健康动物并给予ASU；ART组，患关节炎动物并给予生理盐水；ART/ASU组，患关节炎动物并给予ASU。从手术前7天开始，每天通过灌胃给予溶液，直至实验期结束（种植体植入胫骨后15、30和60天）。通过组织计量学分析（骨与种植体接触率[%BIC]、螺纹间骨面积[%BBT]）和生物力学分析评估种植体的骨整合情况。使用微型计算机断层扫描（micro-CT）分析评估种植体附近的骨体积。进行免疫组织化学分析以评估骨钙素和转化生长因子β1（TGF-β1）的表达。
与ASU组和ART组相比，ART/ASU组在种植体周围区域的骨百分比降低（15天和30天）。与ART组相比，ART/ASU组的去除扭矩值增加（30天），%BIC和%BBT增加（30至60天）。与ART组和CTR组相比，ASU组的%BIC值增加（60天）。CTR组骨钙素表达最高，而ASU组在60天时TGF-β1表达最高。
给予ASU可改善骨整合，尤其是在诱导性关节炎动物中。
