环孢素A减轻短暂性高血糖大鼠缺血再灌注诱导的肾损伤后的细胞凋亡和坏死。

Cyclosporine A attenuates apoptosis and necrosis after ischemia-reperfusion-induced renal injury in transiently hyperglycemic rats.

作者信息

Lemos Sylvio Valença de, Vianna Isabela Galvão, Castiglia Yara Marcondes Machado, Golim Marjorie de Assis, Souza Aparecida Vitória Gonçalves de, Carvalho Lídia Raquel de, Deffune Elenice, Nascimento Paulo do, Módolo Norma Sueli Pinheiro, Vianna Pedro Thadeu Galvão

机构信息

Fellow PhD degree, Postgraduate Program in Anesthesiology, Department of Anesthesiology, Botucatu Medical School, Universidade Estadual de São Paulo (UNESP), Botucatu-SP, Brazil. Conception and design of the study, acquisition of data, manuscript writing, critical revision.

Fellow, Experimental Surgery, Department of Anesthesiology, Botucatu Medical School, UNESP, Botucatu-SP, Brazil. Manuscript writing.

出版信息

Acta Cir Bras. 2017 Mar;32(3):203-210. doi: 10.1590/S0102-865020170030000004.

DOI:10.1590/S0102-865020170030000004

PMID:28403344

Abstract

PURPOSE

: To investigate the effects of cyclosporine A on renal ischemia-reperfusion injury during transient hyperglycemia in rats.

METHODS

: In a model of ischemia-reperfusion-induced renal injury and transiently induced hyperglycemia by intraperitoneal injection of glucose, 2.5 g.kg-1, Wistar rats were anesthetized with either isoflurane or propofol and received intravenous cyclosporine A, 5 mg.kg-1, five minutes before reperfusion. Comparison groups were isoflurane and propofol sham groups and isoflurane and propofol ischemia-reperfusion-induced renal injury. Renal tubular cell viability was quantitatively assessed by flow cytometry after cell culture and classified as early apoptosis, necrotic cells, and intact cells.

RESULTS

: Early apoptosis was significantly higher in isoflurane and propofol anesthetized animals subjected to renal ischemia-reperfusion injury when compared to both cyclosporine A treated and sham groups. Necrosis percentage was significantly higher in propofol-anesthetized animals subjected to renal ischemia-reperfusion injury. The percentage of intact cells was lower in both, isoflurane and propofol anesthetized animals subjected to renal ischemia-reperfusion injury.

CONCLUSION

: In a model of ischemia-reperfusion-induced renal injury, cyclosporine A, 5 m.kg-1, administered five minutes before renal reperfusion in rats with acute-induced hyperglycemia under either isoflurano or propofol anesthesia, attenuated early apoptosis and preserved viability in renal tubular cells, regardless of the anesthetic used.

摘要

目的

研究环孢素A对大鼠短暂高血糖期间肾缺血再灌注损伤的影响。

方法

在通过腹腔注射2.5 g·kg-1葡萄糖诱导缺血再灌注性肾损伤和短暂高血糖的模型中，用异氟烷或丙泊酚麻醉Wistar大鼠，并在再灌注前5分钟静脉注射5 mg·kg-1环孢素A。比较组为异氟烷和丙泊酚假手术组以及异氟烷和丙泊酚缺血再灌注诱导的肾损伤组。细胞培养后通过流式细胞术定量评估肾小管细胞活力，并分为早期凋亡细胞、坏死细胞和完整细胞。