Department of General Surgery and Organ Transplantation, Faculty of Medicine, Kahramanmaras Sutcuimam University, Kahramanmaras 46050, Turkey.
Ren Fail. 2010 Jun;32(5):578-83. doi: 10.3109/08860220903548940.
The purpose of this study was to investigate and compare the efficiency of propofol in the reduction of injury induced by free radicals in a rat model of renal ischemia/reperfusion (I/R).
Twenty-four Wistar rats were divided into four groups in our study. Rats in the sham group underwent laparotomy and were made to wait for 120 min without ischemia. Rats in the control group were given nothing with ischemia-reperfusion. Rats in the I/R groups were given propofol (25 mg/kg) and 10% intralipid (250 mg/kg) ip, respectively, 15 min before the ischemia for 60 min followed by reperfusion for 60 min. The kidney tissues of the rats were taken under anesthesia at the end of the reperfusion period. Evaluation of biochemical malondialdehyde (MDA), superoxide dismutase, and catalase activities and histopathological analysis were performed with these samples.
I/R significantly increased MDA levels (p < 0.05). Histopathological findings of the control group confirmed that there was renal impairment by tubular cell swelling, interstitial edema, medullary congestion, and tubular dilatation. MDA levels were lower in the propofol group compared to control group (p < 0.05). In the propofol group, the level of histopathological scores is significantly decreased than control and intralipid groups in ischemia-reperfusion.
Our results demonstrate that I/R injury was significantly reduced in the presence of propofol. The protective effects of propofol may be due to their antioxidant properties. These results may indicate that propofol anesthesia protects against functional, biochemical, and morphological damage better than control in renal I/R injury.
本研究旨在探讨和比较丙泊酚在减少肾缺血/再灌注（I/R）大鼠模型中自由基损伤的效率。
本研究将 24 只 Wistar 大鼠分为四组。假手术组大鼠仅行剖腹术，等待 120 分钟而不发生缺血。对照组大鼠在发生 I/R 时不给予任何处理。I/R 组大鼠分别在缺血前 15 分钟给予丙泊酚（25mg/kg）和 10%中链脂肪乳（250mg/kg），持续 60 分钟，然后再进行 60 分钟的再灌注。再灌注期末，在麻醉下取大鼠肾脏组织。用这些样本进行生化丙二醛（MDA）、超氧化物歧化酶和过氧化氢酶活性的评估和组织病理学分析。
I/R 显著增加 MDA 水平（p<0.05）。对照组的组织病理学发现证实了肾小管细胞肿胀、间质水肿、髓质充血和肾小管扩张导致的肾功能损害。与对照组相比，丙泊酚组 MDA 水平较低（p<0.05）。在丙泊酚组，与对照组和中链脂肪乳组相比，缺血再灌注后的组织病理学评分明显降低。
我们的结果表明，丙泊酚的存在显著减轻了 I/R 损伤。丙泊酚的保护作用可能与其抗氧化特性有关。这些结果可能表明，丙泊酚麻醉在肾 I/R 损伤中比对照组更能保护肾功能、生化和形态学损伤。
