Fontoura-Andrade José Luiz, Amorim Rivadávio Fernandes Batista de, Sousa João Batista de
Fellow Master degree, Postgraduate Program in Medical Sciences, School of Medicine, Universidade de Brasilia (UnB). DVM, Experimental and Compared Surgery Laboratory, Armed Forces Hospital, Brasilia-DF, Brazil. Acquisition of data, manuscript writing.
PhD, Associate Professor, Department of Pathology, School of Medicine, UnB, Brasilia-DF, Brazil. Manuscript writing, critical revision.
Acta Cir Bras. 2017 Mar;32(3):251-262. doi: 10.1590/S0102-865020170030000010.
: To identify the most relevant flaws in standardization in husbandry practices and lack of transparency to report them. This review proposes some measures in order to improve transparency, reproducibility and eventually external validity in experimental surgery experiments with rat model.
: We performed a search of scientific articles in PUBMED data base. The survey was conducted from august 2016 to January 2017. The keywords used were "reproducibility", "external validity", "rat model", "rat husbandry", "rat housing", and the time frame was up to January 2017. Articles discarded were the ones which the abstract or the key words did not imply that the authors would discuss any relationship of husbandry and housing with the reproducibility and transparency of reporting animal experiment. Reviews and papers that discussed specifically reproducibility and data reporting transparency were laboriously explored, including references for other articles that could fulfil the inclusion criteria. A total of 246 articles were initially found but only 44 were selected.
: Lack of transparency is the rule and not the exception when reporting results with rat model. This results in poor reproducibility and low external validity with the consequence of considerable loss of time and financial resources. There are still much to be done to improve compliance and adherence of researchers, editors and reviewers to adopt guidelines to mitigate some of the challenges that can impair reproducibility and external validity.
: Authors and reviewers should avoid pitfalls of absent, insufficient or inaccurate description of relevant information the rat model used. This information should be correctly published or reported on another source easily available for readers. Environmental conditions are well known by laboratory animal personnel and are well controlled in housing facilities, but usually neglected in experimental laboratories when the rat model is a novelty for the researcher.
识别饲养实践标准化中最相关的缺陷以及缺乏透明度的问题并予以报告。本综述提出了一些措施，以提高使用大鼠模型的实验性外科实验的透明度、可重复性以及最终的外部有效性。
我们在PUBMED数据库中搜索科学文章。调查于2016年8月至2017年1月进行。使用的关键词为“可重复性”“外部有效性”“大鼠模型”“大鼠饲养”“大鼠笼舍”，时间范围截至2017年1月。被舍弃的文章是那些摘要或关键词未暗示作者会讨论饲养和笼舍与动物实验报告的可重复性和透明度之间任何关系的文章。我们费力地查阅了专门讨论可重复性和数据报告透明度的综述及论文，包括其他符合纳入标准的文章的参考文献。最初共找到246篇文章，但仅筛选出44篇。
在报告大鼠模型的结果时，缺乏透明度是常态而非例外。这导致可重复性差和外部有效性低，造成大量时间和财力的损失。在提高研究人员、编辑和审稿人遵守并遵循指南以减轻一些可能损害可重复性和外部有效性的挑战方面，仍有许多工作要做。
作者和审稿人应避免对所使用的大鼠模型相关信息描述缺失、不足或不准确的陷阱。这些信息应正确发表或在读者易于获取的其他来源上报告。环境条件为实验动物人员所熟知，且在饲养设施中得到良好控制，但当大鼠模型对研究人员来说是新事物时，在实验实验室中通常会被忽视。
