Universidade Federal de Pernambuco, Faculdade de Odontologia, Recife, PE, Brasil.
Universidade Federal de Pernambuco, Faculdade de Odontologia, Departamento de Clínica e Odontologia Preventiva, Seção de Patologia Oral Recife, PE, Brasil.
J Appl Oral Sci. 2017 Mar-Apr;25(2):159-167. doi: 10.1590/1678-77572015-0564.
This study aimed to assess the effects of fluoxetine, a selective serotonin reuptake inhibitor, on the formation of the periodontal ligament during pregnancy and lactation in rat pups.
Twelve pregnant rats of Wistar lineage were divided into four study groups. In the control group, 0.9% sodium chloride solution was administered orally, throughout the entire period of the 21 days of pregnancy (CG group) and in the CGL group, it was administrated during pregnancy and lactation (from day 1 of pregnancy to the 21st day after birth). Fluoxetine was administered orally at the dose of 20 mg/kg in a group treated during pregnancy only (FG group), and during pregnancy and lactation (FGL group). Histometrical, histochemical and immunohistochemical analysis of the maxillary first molar periodontium region of the 24 rat pups was made under light microscopy, and periodontal ligament collagen was qualitatively evaluated under a polarizing light microscope.
The quantity of fibroblasts (p=0.006), osteoblasts (p=0.027) and cementoblasts (p=0.001) was reduced in pups from the rats that received fluoxetine during pregnancy and lactation. No alterations were seen in the collagen fibers.
These findings suggest that periodontal tissue may be sensitive to fluoxetine, and its interference in reducing periodontal cells depends on exposure time during lactation.
本研究旨在评估选择性 5-羟色胺再摄取抑制剂氟西汀对妊娠和哺乳期大鼠幼仔牙周韧带形成的影响。
12 只 Wistar 系怀孕大鼠被分为 4 个研究组。在对照组中，整个 21 天的妊娠期间（CG 组）经口给予 0.9%氯化钠溶液，在 CGL 组中，从妊娠第 1 天到出生后第 21 天给予该溶液。仅在妊娠期间（FG 组）和妊娠及哺乳期（FGL 组）给予氟西汀，剂量为 20mg/kg。对 24 只大鼠的上颌第一磨牙牙周区进行组织学、组织化学和免疫组织化学分析，并在偏光显微镜下定性评估牙周韧带胶原。
接受氟西汀治疗的妊娠和哺乳期大鼠的幼仔中，成纤维细胞（p=0.006）、成骨细胞（p=0.027）和牙骨质细胞（p=0.001）的数量减少。胶原纤维没有变化。
这些发现表明牙周组织可能对氟西汀敏感，其对牙周细胞的干扰取决于哺乳期的暴露时间。
