阿米替林对白化病大鼠牙周组织影响的组织学、免疫组织化学及影像学评估（一项实验研究）

Histological, immunohistochemical and radiographic evaluation of Amitriptyline administration on the periodontium of albino rats (An experimental study).

作者信息

Hassan Rabab, Ahmed Naglaa Fathallah, Hussein Safaa Ismail

机构信息

Associate professor of Oral Biology, Faculty of Dentistry, Ain Shams University, Cairo, Egypt.

Lecturer of Oral Radiology, Faculty of Dentistry, Ain Shams University, Cairo, Egypt.

出版信息

Saudi Dent J. 2022 Sep;34(6):449-457. doi: 10.1016/j.sdentj.2022.06.010. Epub 2022 Jul 4.

DOI:10.1016/j.sdentj.2022.06.010

PMID:36092527

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9453502/

Abstract

BACKGROUND

Amitriptyline is a tricyclic antidepressant drug accustomed to treat depressive disorders. It recorded many side effects on different tissues.

OBJECTIVE

To investigate reaction of Albino rats' periodontium after oral administration of Amitriptyline histologically and radiographically.

METHODS

Fourteen adult male albino rats (150-200 g) were divided into two groups, control and experimental. Rats of experimental group received 10 mg⁄kg⁄day of Amitriptyline hydrochloride by oral gavage for four weeks. Mandibles were prepared for hematoxylin and eosin (H&E) and anti-osteopontin (Anti-OPN) immunohistochemistry staining. Bone mineral density was measured in mandibular alveolar bone. Statistical analysis for Anti-OPN and relative Hounsfield unit value (HU value) was performed using independent-samples -test.

RESULTS

Gingiva of experimental group showed epithelial degeneration with pyknotic nuclei and disintegration in lamina propria. Areas of separation in alveolar bone and degeneration of some regions in cementum were seen with apparent increase in periodontal ligament (PDL) thickness and its detachment from bone and cementum at some regions. Immunohistochemical examination of experimental group showed apparently increased immunopositivity in gingiva, cementocytes, osteocytes, cementum, bone matrices, fibroblasts and PDL fibers when compared to control group. Statistical analysis revealed insignificant difference of Anti-OPN area% in gingiva between both studied groups. While there was statistical significant increase of Anti-OPN area% in the other periodontium tissues and high statistical significant decrease of relative HU value in experimental group when compared to control.

CONCLUSIONS

Amitriptyline has destructive effect on periodontal tissues and statistically increases the expression of Anti-OPN in all periodontal tissues except gingiva and decreases bone mineral density.

摘要

背景

阿米替林是一种用于治疗抑郁症的三环类抗抑郁药。它对不同组织有许多副作用。

目的

通过组织学和影像学研究白化大鼠口服阿米替林后牙周组织的反应。

方法

将14只成年雄性白化大鼠（150 - 200克）分为两组，即对照组和实验组。实验组大鼠通过口服灌胃给予10毫克/千克/天的盐酸阿米替林，持续四周。制备下颌骨用于苏木精和伊红（H&E）染色以及抗骨桥蛋白（Anti - OPN）免疫组织化学染色。测量下颌牙槽骨的骨密度。使用独立样本t检验对Anti - OPN和相对亨氏单位值（HU值）进行统计分析。

结果

实验组牙龈显示上皮变性，细胞核固缩，固有层崩解。可见牙槽骨分离区域以及牙骨质某些区域的变性，牙周膜（PDL）厚度明显增加，且在某些区域与骨和牙骨质分离。实验组的免疫组织化学检查显示，与对照组相比，牙龈、牙骨质细胞、骨细胞、牙骨质、骨基质、成纤维细胞和PDL纤维中的免疫阳性明显增加。统计分析显示，研究的两组之间牙龈中Anti - OPN面积百分比无显著差异。而与对照组相比，实验组其他牙周组织中Anti - OPN面积百分比有统计学显著增加，相对HU值有高度统计学显著降低。