Lau Show Xuan, Leong Yin Yee, Ng Wai Hoe, Ng Albert Wee Po, Ismail Ida Shazrina, Yusoff Narazah Mohd, Ramasamy Rajesh, Tan Jun Jie
Advanced Medical and Dental Institute, UniversitiSains Malaysia, Bertam 13200 Kepala Batas, Penang, Malaysia.
Immunology Unit, Department of Pathology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang, 43400, Selangor Darul Ehsan, Malaysia.
Cell Biol Int. 2017 Jun;41(6):697-704. doi: 10.1002/cbin.10774. Epub 2017 May 3.
Studies showed that co-transplantation of mesenchymal stem cells (MSCs) and cord blood-derived CD34 hematopoietic stem cells (HSCs) offered greater therapeutic effects but little is known regarding the effects of human Wharton's jelly derived MSCs on HSC expansion and red blood cell (RBC) generation in vitro. This study aimed to investigate the effects of MSCs on HSC expansion and differentiation. HSCs were co-cultured with MSCs or with 10% MSCs-derived conditioned medium, with HSCs cultured under standard medium served as a control. Cell expansion rates, number of mononuclear cell post-expansion and number of enucleated cells post-differentiation were evaluated. HSCs showed superior proliferation in the presence of MSC with mean expansion rate of 3.5 × 10 ± 1.8 × 10 after day 7 compared to the conditioned medium and the control group (8.9 × 10 ± 1.1 × 10 and 7.0 × 10 ± 3.3 × 10 respectively, P < 0.001). Although no significant differences in RBC differentiation were observed between groups at passage IV, the number of enucleated cell was greater compared to earlier passages, indicating successful RBC differentiation. Cord blood-derived CD34 HSCs can be greatly expanded by co-culturing with MSCs without affecting the RBC differentiation capability, suggesting the importance of direct MSC-HSCs contact in HSC expansion and RBC differentiation.
研究表明，间充质干细胞（MSCs）与脐带血来源的CD34造血干细胞（HSCs）共移植具有更大的治疗效果，但关于人脐带华通氏胶来源的MSCs对体外HSC扩增和红细胞（RBC）生成的影响知之甚少。本研究旨在探讨MSCs对HSC扩增和分化的影响。将HSCs与MSCs或10% MSCs条件培养基共培养，以在标准培养基中培养的HSCs作为对照。评估细胞扩增率、扩增后单核细胞数量和分化后去核细胞数量。与条件培养基和对照组相比（分别为8.9×10±1.1×10和7.0×10±3.3×10），HSCs在与MSCs共培养时显示出更好的增殖能力，第7天后平均扩增率为3.5×10±1.8×10（P<0.001）。虽然在第IV代时各组之间在RBC分化方面未观察到显著差异，但与早期传代相比，去核细胞数量更多，表明RBC分化成功。脐带血来源的CD34 HSCs与MSCs共培养可显著扩增，且不影响RBC分化能力，提示MSCs与HSCs直接接触在HSC扩增和RBC分化中具有重要作用。
