Talevska Aleksandra, Pejin Boris, Kojic Vesna, Beric Tanja, Stankovic Slavisa
a Faculty of Biology , University of Belgrade , Belgrade , Serbia.
b Department of Life Sciences , Institute for Multidisciplinary Research - IMSI, University of Belgrade , Belgrade , Serbia.
Nat Prod Res. 2018 Mar;32(5):568-571. doi: 10.1080/14786419.2017.1315719. Epub 2017 Apr 13.
In vitro anti-tumour and anti-radical activities of the acetone extract of the freshwater sponge Ochridaspongia rotunda were the subject of this study. The extract was found to be highly cytotoxic to human lung tumour cell line A-549 reaching IC value of 5.01 ± 0.21 μg/mL. Indeed, it displayed only 2-fold less anti-tumour activity than doxorubicin (IC value 2.42 ± 0.13 μg/mL) used as a positive control. The same extract was also found to be almost 37-fold more selective against A-549 vs. MRC-5 (normal) lung cells, in difference to weak selectivity of doxorubicin (less than 3-fold). Its profound anti-DPPH radical activity comparable to that of quercetin (IC values 3.68 ± 0.19 and 3.14 ± 0.09 μg/mL, respectively) coupled with no signs of genotoxicity in the comet assay (MRC-5 cell line, vs. doxorubicin) has actually implicated the importance of this animal bioresource in searching for pharmaceutically useful bioactive compounds of natural origin.
本研究以淡水海绵圆奥氏海绵丙酮提取物的体外抗肿瘤和抗自由基活性为主题。该提取物对人肺癌细胞系A-549具有高度细胞毒性，IC值为5.01±0.21μg/mL。实际上，其抗肿瘤活性仅比用作阳性对照的阿霉素（IC值2.42±0.13μg/mL）低2倍。还发现相同提取物对A-549细胞与MRC-5（正常）肺细胞的选择性几乎高37倍，这与阿霉素的弱选择性（小于3倍）不同。其与槲皮素相当的显著抗DPPH自由基活性（IC值分别为3.68±0.19和3.14±0.09μg/mL），以及彗星试验（MRC-5细胞系，与阿霉素相比）中无遗传毒性迹象，实际上暗示了这种动物生物资源在寻找天然来源的药用生物活性化合物方面的重要性。
