Qi Donglai, Li Qian, Wang Pei, Wang Xiang
a School of Environment and Chemical Engineering, Tianjin Polytechnic University , Tianjin , P.R. China.
Artif Cells Nanomed Biotechnol. 2017 Sep;45(6):1-5. doi: 10.1080/21691401.2017.1309659. Epub 2017 Apr 12.
Haemoglobin site-specifically modified with ferulic acid (FA-Hb), was synthesized. The effects on the characteristic absorption and the oxygen saturation curve of FA-Hb were investigated by UV-Vis spectrophotometry and oxygen analyzer. Furthermore, the autoxidation rates of Hb and FA-Hb were also discussed with or without sodium azide in the system. The results showed that there was no obvious transformation of the heme structure of FA-Hb. It was also demonstrated that the oxygen-carrying capacity of FA-Hb was similar to the natural Hb, but the autoxidation rate of FA-Hb was much lower than Hb in different systems.
合成了用阿魏酸进行位点特异性修饰的血红蛋白（FA-Hb）。通过紫外可见分光光度法和氧分析仪研究了FA-Hb对特征吸收和氧饱和曲线的影响。此外，还讨论了体系中有无叠氮化钠时Hb和FA-Hb的自氧化速率。结果表明，FA-Hb的血红素结构没有明显转变。还证明了FA-Hb的携氧能力与天然Hb相似，但在不同体系中FA-Hb的自氧化速率远低于Hb。
