a School of Environmental and Chemical Engineering, Tianjin Polytechnic University , Tianjin , P. R. China.
Artif Cells Nanomed Biotechnol. 2018;46(sup1):1191-1199. doi: 10.1080/21691401.2018.1448987. Epub 2018 Mar 13.
During the development of artificial red blood cell (RBC) substitutes, oxidation side reaction is one of the major factors that hinder the application of haemoglobin (Hb)-based oxygen carriers (HBOCs). In order to avoid oxidation toxicity, we designed and prepared natural Hb conjugated with ferulic acid (FA) via simple chemical modification. In addition, the thiol groups on Hb surface were increased via the reaction of Hb with 2-iminothiolane (2-IT) and then modified with FA for the study of anti-oxidant ability. It was showed that Hb modified with FA (FA-Hb) had similar oxygen-binding capacity to natural Hb. Moreover, the anti-oxidant ability of FA-Hb in vitro in different systems was superior to natural Hb and in proportion to the degree of modification of FA. The results indicate that FA-Hb might have the potential to serve as a novel oxygen carrier with the capacity to reduce oxidative side reaction.
在人工红细胞（RBC）代用品的开发过程中，氧化副反应是阻碍基于血红蛋白（Hb）的氧载体（HBOCs）应用的主要因素之一。为了避免氧化毒性，我们通过简单的化学修饰设计并制备了与阿魏酸（FA）偶联的天然 Hb。此外，通过 Hb 与 2-亚氨基硫代烷（2-IT）的反应增加了 Hb 表面的巯基，然后用 FA 对其进行修饰，以研究其抗氧化能力。结果表明，FA 修饰的 Hb（FA-Hb）具有与天然 Hb 相似的氧结合能力。此外，FA-Hb 在不同体系中的体外抗氧化能力优于天然 Hb，且与 FA 修饰程度成正比。结果表明，FA-Hb 可能具有作为一种新型氧载体的潜力，能够减少氧化副反应。
