Harbauer G, Allendorf A
Department of Experimental Surgery, Saar University, Homburg/Saar, FRG.
Haemostasis. 1988;18 Suppl 3:69-72. doi: 10.1159/000215870.
6 different preparations of low-molecular-weight heparin (LMWH) derivatives and 1 unfractionated heparin (UFH) were tested for their thromboprophylactic effects in an experimental model which closely simulates clinical conditions. Each preparation was tested in 5 rabbits 2 h and in 5 rabbits 12 h after subcutaneous application of 20-200 U/kg body weight (according to the suggestions of the manufacturers). The animals were anesthetized with intravenous pentobarbital sodium about 90 min or 11.5 h after the application of the heparin preparation. The right external jugular vein was dissected free and a thrombogenesis was provoked. There was no significant protection against occluding thrombosis 2 h after LMWH or UFH injection, when plasma levels of heparin-like activities were found to be high in another experimental series. Surprisingly, there were definite thromboprophylactic effects 12 h (when heparin-like plasma activities had disappeared) after application of 4 (out of 6) LMWH preparations. No protection against thrombosis was seen 12 h after application of UFH.
在一个紧密模拟临床情况的实验模型中，对6种不同的低分子量肝素（LMWH）衍生物制剂和1种普通肝素（UFH）进行了血栓预防作用测试。按照制造商的建议，以20 - 200 U/kg体重的剂量对每组5只兔子进行皮下注射后，分别在2小时和12小时对每种制剂进行测试。在注射肝素制剂约90分钟或11.5小时后，用静脉注射戊巴比妥钠对动物进行麻醉。解剖分离出右侧颈外静脉并诱发血栓形成。在另一个实验系列中，当发现肝素样活性的血浆水平较高时，注射LMWH或UFH后2小时对闭塞性血栓形成没有显著的预防作用。令人惊讶的是，在应用6种LMWH制剂中的4种后12小时（此时肝素样血浆活性已经消失）有明确的血栓预防作用。注射UFH后12小时未观察到对血栓形成的预防作用。
