Wu X-Q, Wang Y-Q, Xu S-M, Liu J-F, Bi X-Y, Wang Z-Q, Zhang J-P
Center of Reproductive Medicine, Shanxi Women and Children Health Hospital, No. 13, Xinmin North Street, Xinghualing District, 030013, Taiyuan, Shanxi, China.
Center of Reproductive Medicine, Shanxi Women and Children Health Hospital, No. 13, Xinmin North Street, Xinghualing District, 030013, Taiyuan, Shanxi, China.
J Gynecol Obstet Hum Reprod. 2017 Jan;46(1):93-99. doi: 10.1016/j.jgyn.2015.08.013. Epub 2015 Oct 21.
Investigate the expressions of WNTs in granulosa cells of PCOS in North China, and explore the possible mechanism.
Patients with PCOS (n=40) and controls without PCOS (n=20) were enrolled into the study. The levels of serum sex hormone were detected by chemiluminescent enzyme immunoassay. RT-qPCR was used to measure mRNA levels of WNT family members (WNT1, WNT3, WNT4, WNT5, sFRP4 and sFRP5). The levels of Bax and Bcl-2 were measured by enzyme-linked immunosorbent assay.
The levels of PROG, TES and LH/FSH had difference (p<0.05) on third day of menstrual cycle between PCOS and control groups. After injection of HCG, the levels of LH, E and PROG had significant difference (p<0.05). The PCOS group had higher transcript levels of WNT1, WNT3 and WNT4 (p<0.05), compared to controls. The level of secreted frizzled-related protein (sFRP4) was lower level in PCOS patients (p<0.05). In addition, we found a significant reduction of the β-catenin as well as the downstream targets (survivin and BMP4) (p<0.05). The level of Bax was significantly higher in PCOS group than in the control group (p<0.05). Above all, the WNT/β-catenin signaling pathway might be involved into granulosa cell apoptosis, which may provide a strategy for clinical treatment.
研究华北地区多囊卵巢综合征（PCOS）患者颗粒细胞中WNTs的表达情况，并探讨其可能的机制。
本研究纳入了40例PCOS患者和20例无PCOS的对照者。采用化学发光酶免疫分析法检测血清性激素水平。运用RT-qPCR检测WNT家族成员（WNT1、WNT3、WNT4、WNT5、sFRP4和sFRP5）的mRNA水平。通过酶联免疫吸附测定法检测Bax和Bcl-2的水平。
PCOS组与对照组在月经周期第3天的PROG、TES和LH/FSH水平存在差异（p<0.05）。注射HCG后，LH、E和PROG水平有显著差异（p<0.05）。与对照组相比，PCOS组WNT1、WNT3和WNT4的转录水平更高（p<0.05）。PCOS患者的分泌型卷曲相关蛋白（sFRP4）水平较低（p<0.05）。此外，我们发现β-连环蛋白及其下游靶点（生存素和BMP4）显著降低（p<0.05）。PCOS组的Bax水平显著高于对照组（p<0.05）。综上所述，WNT/β-连环蛋白信号通路可能参与颗粒细胞凋亡，这可能为临床治疗提供一种策略。
