Chermuła Błażej, Kranc Wiesława, Celichowski Piotr, Stelmach Bogusława, Piotrowska-Kempisty Hanna, Mozdziak Paul, Pawelczyk Leszek, Spaczyński Robert Zygmunt, Kempisty Bartosz
Department of Gynecology, Division of Infertility and Reproductive Endocrinology, Obstetrics and Gynecological Oncology, Poznan University of Medical Sciences, 33 Polna St., 60-535 Poznan, Poland.
Department of Anatomy, Poznan University of Medical Sciences, 6 Swiecickiego St., 60-781 Poznan, Poland.
J Clin Med. 2021 Dec 24;11(1):73. doi: 10.3390/jcm11010073.
In the growing ovarian follicle, the maturing oocyte is accompanied by cumulus (CCs) and granulosa (GCs) cells. Currently, there remain many unanswered questions about the epithelial origin of these cells. Global and targeted gene transcript levels were assessed on 1, 7, 15, 30 days of culture for CCs and GCs. Detailed analysis of the genes belonging to epithelial cell-associated ontological groups allowed us to assess a total of 168 genes expressed in CCs (97 genes) and GCs (71 genes) during long-term in vitro culture. Expression changes of the analyzed genes allowed the identification of the group of genes: TGFBR3, PTGS2, PRKX, AHI1, and IL11, whose expression decreased the most and the group of ANXA3, DKK1, CCND1, STC1, CAV1, and SFRP4 genes, whose expression significantly increased. These genes' expression indicates CCs and GCs epithelialization processes and their epithelial origin. Expression change analysis of genes involved in epithelization processes in GCs and CCs during their in vitro culture made it possible to describe the most significantly altered of the 11 genes. Detailed analysis of gene expression in these two cell populations at different time intervals confirms their ovarian surface epithelial origin. Furthermore, some gene expression profiles appear to have tumorigenic properties, suggesting that granulosa cells may play a role in cancerogenesis.
在生长中的卵巢卵泡中，成熟的卵母细胞伴有卵丘（CCs）和颗粒（GCs）细胞。目前，关于这些细胞的上皮起源仍有许多未解决的问题。在培养的第1、7、15、30天对CCs和GCs的全局和靶向基因转录水平进行了评估。对属于上皮细胞相关本体组的基因进行详细分析，使我们能够评估在长期体外培养期间在CCs（97个基因）和GCs（71个基因）中表达的总共168个基因。分析基因的表达变化使得能够鉴定出表达下降最多的一组基因：TGFBR3、PTGS2、PRKX、AHI1和IL11，以及表达显著增加的一组基因：ANXA3、DKK1、CCND1、STC1、CAV1和SFRP4基因。这些基因的表达表明CCs和GCs的上皮化过程及其上皮起源。对GCs和CCs在体外培养期间参与上皮化过程的基因的表达变化分析使得能够描述11个基因中变化最显著的基因。对这两个细胞群体在不同时间间隔的基因表达进行详细分析，证实了它们的卵巢表面上皮起源。此外，一些基因表达谱似乎具有致瘤特性，这表明颗粒细胞可能在癌症发生中起作用。
