Department of Gastroenterology, University of Ulsan College of Medicine, Asan Medical Center, Seoul, South Korea.
Department of Internal Medicine, Division of Gastroenterology, University of Michigan, Ann Arbor, MI, USA; Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI, USA; Department of Mechanical Engineering, University of Michigan, Ann Arbor, MI, USA.
Lancet Gastroenterol Hepatol. 2016 Oct;1(2):147-155. doi: 10.1016/S2468-1253(16)30027-9. Epub 2016 Sep 8.
Endoscopy uses optical imaging methods to investigate tissue in a non-destructive manner with high resolution over a broad range of wavelengths, thus providing a powerful tool to rapidly visualise mucosal surfaces in the digestive tract. Molecular imaging is an important advancement that has been clinically demonstrated for early cancer detection and guidance of therapy. With this approach, imaging can be used to observe expression patterns of molecular targets to improve understanding of key biological mechanisms that drive disease progression. Prototype devices that collect fluorescence for wide-field or microscopic images have been developed. Several targeting moieties, including enzyme-activatable probes, antibodies, peptides, and lectins, have been administered in preclinical and clinical imaging studies in vivo. These emerging technologies provide useful approaches to study molecular events in different signalling pathways, producing insights that could lead to improved interventions to prevent and treat gastrointestinal diseases. In this Review, we introduce the basic concepts that form the foundation for development of molecular endoscopy and summarise key results from preclinical and clinical studies.
内窥镜使用光学成像方法，在广泛的波长范围内以高分辨率非破坏性地研究组织，从而提供了一种快速可视化消化道黏膜表面的强大工具。分子成像技术是一种重要的进步，已在临床上用于早期癌症检测和治疗指导。通过这种方法，可以利用成像来观察分子靶标的表达模式，以提高对驱动疾病进展的关键生物学机制的理解。已经开发出用于收集宽场或显微镜图像的荧光的原型设备。在体内的临床前和临床成像研究中，已经给予了几种靶向部分，包括酶激活探针、抗体、肽和凝集素。这些新兴技术为研究不同信号通路中的分子事件提供了有用的方法，为改善预防和治疗胃肠道疾病的干预措施提供了见解。在这篇综述中，我们介绍了构成分子内窥镜发展基础的基本概念，并总结了临床前和临床研究的关键结果。
