Taylor P M, Luna Spl, Dye A
Department of Clinical Veterinary Medicine, University of Cambridge, UK.
Faculty of Veterinary Medicine and Animal Science, UNESP, Campus de Botucatu, CEP 18618-000, Botucatu, São Paulo, Brazil.
Vet Anaesth Analg. 2000 Jan;27(1):27-35. doi: 10.1046/j.1467-2995.2000.00004.x. Epub 2016 Nov 15.
To determine the effects of surgery, hypoxia, hypercapnia and flunixin administration on plasma β-endorphin immunoreactivity (BEI) in anaesthetized horses.
Prospective crossover study.
Six healthy adult Welsh Mountain ponies and seven healthy adult Thoroughbreds.
Ponies were anaesthetized with thiopentone and halothane or with pentobarbitone and the horses with guaiphenesin, thiopentone and halothane. Ponies were anaesthetized for 2 hours and on separate occasions underwent a period of hypoxia, hypercapnia, anaesthesia only, or were given flunixin at induction. The horses were anaesthetized for 2 hours and on separate occasions underwent surgery to relocate one carotid artery subcutaneously or anaesthesia only. Plasma samples were taken pre-anaesthesia, at 20 minute intervals during, and after anaesthesia for BEI assay using radio-immunoassay. Analysis of variance of the concentration-time curve was used for statistical analysis.
Pre-anaesthetic β-endorphin immunoreactivity (BEI) values ranged between 5.7 and 20.4 pmol L Induction of anaesthesia caused a five to 10 fold increase in mean plasma BEI in all cases except the hypercapnia group. Halothane anaesthesia increased BEI in ponies and horses but there were no significant changes during pentobarbitone anaesthesia. The increase in BEI in the hypoxic group was greater (peak value 136.8 ± 32.2 pmol L) and sustained for a longer period compared with levels in those given halothane alone or in those which became hypercapnic. There was marked individual variation in the flunixin group and changes were not significant. Surgery in the horses resulted in the highest peak values in the study (182.5 ± 153.0 pmol L) but the AUC was not significantly higher than in the same animals without surgery, where the peak value was 102.9 ± 42.1 pmol L CONCLUSIONS: Beta-endorphin appeared to be a sensitive marker of an endocrine stress response but its physiological role during equine anaesthesia is unknown.
Unknown.
确定手术、低氧、高碳酸血症及氟尼辛给药对麻醉马匹血浆β-内啡肽免疫反应性（BEI）的影响。
前瞻性交叉研究。
6匹健康成年威尔士山地矮种马和7匹健康成年纯种马。
矮种马用硫喷妥钠和氟烷或戊巴比妥麻醉，马匹用愈创甘油醚、硫喷妥钠和氟烷麻醉。矮种马麻醉2小时，在不同时间分别经历一段低氧、高碳酸血症、仅麻醉阶段，或在诱导时给予氟尼辛。马匹麻醉2小时，在不同时间分别接受皮下重新定位一条颈动脉的手术或仅接受麻醉。在麻醉前、麻醉期间每隔20分钟及麻醉后采集血浆样本，用放射免疫分析法进行BEI检测。浓度-时间曲线的方差分析用于统计分析。
麻醉前β-内啡肽免疫反应性（BEI）值在5.7至20.4 pmol/L之间。除高碳酸血症组外，所有情况下麻醉诱导均使平均血浆BEI升高5至10倍。氟烷麻醉使矮种马和马匹的BEI升高，但戊巴比妥麻醉期间无显著变化。与仅接受氟烷麻醉或发生高碳酸血症的马匹相比，低氧组BEI升高幅度更大（峰值136.8±32.2 pmol/L）且持续时间更长。氟尼辛组存在明显个体差异，变化不显著。马匹手术导致研究中最高峰值（182.5±153.0 pmol/L），但其曲线下面积（AUC）并不显著高于未手术的同一批动物，未手术时峰值为102.9±42.1 pmol/L。结论：β-内啡肽似乎是内分泌应激反应的敏感标志物，但其在马麻醉期间的生理作用尚不清楚。
未知。
