Zhao Chengcheng, Li Xia, Miao Jing, Jing Songsong, Li Xuejiao, Huang Luqi, Gao Wenyuan
Tianjin Key Laboratory for Modern Drug Delivery & High-Efficiency, School of Pharmaceutical Science and Technology, Tianjin University, No. 92 Weijin Road, Nankai District, Tianjin 300072, China.
Tianjin Key Laboratory for Modern Drug Delivery & High-Efficiency, School of Pharmaceutical Science and Technology, Tianjin University, No. 92 Weijin Road, Nankai District, Tianjin 300072, China.
Int J Biol Macromol. 2017 Sep;102:847-856. doi: 10.1016/j.ijbiomac.2017.04.031. Epub 2017 Apr 9.
The rhizoma of Dioscorea hemsleyi (DH) has been used as a treatment of diabetes in China for hundreds of years. Polysaccharides in DH were extracted by using ultrasonic-assisted extraction (UAE), cold water extraction (CWE), warm water extraction (WWE) and hot water extraction (HWE), separately. Then the different characterizations of four DH polysaccharide (DHP) samples were analyzed by high-performance liquid chromatography (HPLC), high-performance Gel permeation chromatography (HGPC), ultraviolet-visible spectroscopy(UV), fourier transform-infrared spectroscopy (FT-IR) and scanning electron microscopy (SEM). Their activities in vitro of DHP were compared. Experimental results showed that HWE had the highest yield and large molecular weight. CWE had the highest uronic acid yield and little molecular weight, and its DPPH, AGI and AAI activity were the best. The molecular weight of UAE was small, and its RP and FRAP activity were the best. Four DHP samples had differences in the surface topography, while they all had the typical IR spectra characteristic of polysaccharides. According the correlation analysis, it showed that the more uronic acid and the lower molecular weight was, the higher the antioxidant activity was. The high content of monosaccharide composition of Xyl, Ara, GlcA and GalA, and little molecular weight have good effect on antidiabetic activity.
绵萆薢根茎在中国已被用于治疗糖尿病数百年。分别采用超声辅助提取（UAE）、冷水提取（CWE）、温水提取（WWE）和热水提取（HWE）法从绵萆薢中提取多糖。然后通过高效液相色谱（HPLC）、高效凝胶渗透色谱（HGPC）、紫外可见光谱（UV）、傅里叶变换红外光谱（FT-IR）和扫描电子显微镜（SEM）对四个绵萆薢多糖（DHP）样品的不同特性进行分析。比较了它们在体外的活性。实验结果表明，热水提取法的得率最高且分子量较大。冷水提取法的糖醛酸得率最高且分子量较小，其对二苯基苦味酰基自由基（DPPH）、α-葡萄糖苷酶抑制（AGI）和淀粉酶抑制（AAI）活性最佳。超声辅助提取法得到的多糖分子量较小，其还原力（RP）和铁离子还原抗氧化能力（FRAP）活性最佳。四个DHP样品的表面形貌存在差异，但它们都具有多糖典型的红外光谱特征。相关性分析表明，糖醛酸含量越高、分子量越低，抗氧化活性越高。木糖（Xyl）、阿拉伯糖（Ara）、葡萄糖醛酸（GlcA）和半乳糖醛酸（GalA）单糖组成含量高且分子量小，对降糖活性有良好作用。
