Department of Food Science and Technology, Pukyong National University, 45 Yongso-ro, Nam-Gu, Busan 48513, Korea; School of Chemical and Bioengineering, Addis Ababa Institute of Technology, Addis Ababa University, P.O. Box 385, Addis Ababa, Ethiopia.
Department of Food Science and Technology, Pukyong National University, 45 Yongso-ro, Nam-Gu, Busan 48513, Korea.
Int J Biol Macromol. 2018 Apr 1;109:711-719. doi: 10.1016/j.ijbiomac.2017.12.120. Epub 2017 Dec 30.
In this study, we used a novel approach to recover polysaccharide from spent coffee ground (SCG) by combining pretreatments and subcritical water hydrolysis (SCWH). The independent variables which affect SCWH were optimized using response surface methodology. The highest yield of SCG polysaccharides (SCGPSs) (18.25 ± 0.21%) was obtained using ultrasonic pretreatment and SCWH conditions of temperature (178.85 °C), pressure (20 bar), and extraction time (5 min). The extracted SCGPSs showed high antioxidant activity as measured using ABTS and DPPH radical scavenging assay with IC values of 1.83 ± 0.03 and 2.66 ± 0.13 mg/ml respectively. SCGPSs also showed in vitro hypoglycemic activities. The structural and thermal characterization of the polysaccharide showed that the extracted polysaccharide has a typical carbohydrate features. The results of this study suggested that the extracted polysaccharide could have a potential application in food and related industries.
在这项研究中，我们采用了一种新的方法，通过结合预处理和亚临界水水解（SCWH）从废咖啡渣（SCG）中回收多糖。使用响应面法优化了影响 SCWH 的独立变量。在超声预处理和 SCWH 条件为温度（178.85°C）、压力（20 巴）和提取时间（5 分钟）下，获得了最高的 SCG 多糖（SCGPSs）收率（18.25±0.21%）。提取的 SCGPSs 表现出高的抗氧化活性，使用 ABTS 和 DPPH 自由基清除测定法测定的 IC 值分别为 1.83±0.03 和 2.66±0.13 mg/ml。SCGPSs 还表现出体外降血糖活性。多糖的结构和热特性表明，提取的多糖具有典型的碳水化合物特征。本研究结果表明，提取的多糖在食品和相关行业可能具有潜在的应用。
