Ma Yao, Chen Wan-Jun, Li Zhao-Hui, Zhang Feng, Gao Yan, Luan Yun-Xia
Key Laboratory of Insect Developmental and Evolutionary Biology Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai China; University of Chinese Academy of Sciences Beijing China.
Key Laboratory of Insect Developmental and Evolutionary Biology Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai China.
Ecol Evol. 2017 Feb 10;7(7):2009-2017. doi: 10.1002/ece3.2738. eCollection 2017 Apr.
The endosymbiont has been detected in a few parthenogenetic collembolans sampled in Europe and America, including three species of Poduromorpha, two species of Entomobryomorpha, and two species of Neelipleona. Based on 16S rRNA and gene sequences, most of the infecting parthenogenetic collembolans were characterized as members of supergroup E and showed concordant phylogeny with their hosts. However, the two neelipleonan symbionts form another unique group, indicating that has infected parthenogenetic collembolans multiple times. In this study, five parthenogenetic collembolan species were identified as hosts of , and four new strains were reported for four collembolan species sampled in China, respectively, including a neelipleonan strain from (Minc). Our results demonstrated that the multilocus sequence typing (MLST) system is superior to the 16S rRNA + approach for phylogenetic analyses of collembolan . The MLST system assigned these of parthenogenetic collembolans to supergroup E as a unique clade, which included Minc, supporting the monophyletic origin of in parthenogenetic collembolan species. Moreover, our data suggested supergroup E as one of the most divergent lineages in and revealed the discrepancy between the phylogenies of from parthenogenetic collembolans and their hosts, which may result from the high level of genetic divergence between collembolan , in association with the geographic differentiation of their hosts, or the possible horizontal transmission of between different collembolan species.
在内共生菌已在欧洲和美洲采集的一些孤雌生殖弹尾虫中被检测到，包括三种原等节类、两种长角跳虫类和两种短角圆跳虫类。基于16S rRNA和基因序列，感染孤雌生殖弹尾虫的大多数内共生菌被鉴定为E超群的成员，并与其宿主显示出一致的系统发育关系。然而，两种短角圆跳虫类内共生菌形成了另一个独特的群体，这表明内共生菌已多次感染孤雌生殖弹尾虫。在本研究中，五种孤雌生殖弹尾虫物种被鉴定为内共生菌的宿主，并且分别报道了在中国采集的四种弹尾虫物种的四个新内共生菌菌株，包括来自（闽）的一个短角圆跳虫类菌株。我们的结果表明，多位点序列分型（MLST）系统在弹尾虫内共生菌的系统发育分析中优于16S rRNA + 方法。MLST系统将这些孤雌生殖弹尾虫的内共生菌归为E超群的一个独特分支，其中包括闽，支持了内共生菌在孤雌生殖弹尾虫物种中的单系起源。此外，我们的数据表明E超群是内共生菌中最具分歧的谱系之一，并揭示了孤雌生殖弹尾虫内共生菌与其宿主的系统发育之间的差异，这可能是由于弹尾虫内共生菌之间的高度遗传分歧，以及其宿主的地理分化，或者内共生菌在不同弹尾虫物种之间可能的水平传播所致。
