Reichardt J K, Berg P
Department of Biochemistry, Stanford University School of Medicine, CA 94305.
Mol Biol Med. 1988 Apr;5(2):107-22.
We report the cloning and characterization of a cDNA that encodes a functional human galactose-1-phosphate uridyl transferase (GALT). The cDNA is 1400 bases in length and encodes a 43,000 Mr protein. The cloning strategy involved the identification of short peptide sequences conserved between the homologous enzymes from Escherichia coli and yeast, and the construction of oligonucleotide pools corresponding to the conserved patches. These patches of conserved amino acids tend to be conserved in humans as well.
我们报道了一个编码功能性人半乳糖-1-磷酸尿苷酰转移酶（GALT）的cDNA的克隆及特性分析。该cDNA长度为1400个碱基，编码一个分子量为43,000的蛋白质。克隆策略包括鉴定大肠杆菌和酵母同源酶之间保守的短肽序列，以及构建与保守片段对应的寡核苷酸库。这些保守氨基酸片段在人类中也往往是保守的。
