Strain improvement of Lentzea sp. 7887 for higher yield per unit volume on hydroxylation of cyclosporine derivative FR901459.
作者信息
Yabutani Tetsuya, Tsujimoto Mami, Ohira Shunsuke, Shimizu Shiho, Nakano Hideo
机构信息
a Technology Product Management , Astellas Pharma Inc. , Tokyo , Japan.
d Laboratory of Molecular Biotechnology, Division of Biotechnology, Department of Bioengineering Science , Graduate School of Bioagricultural Sciences, Nagoya University , Nagoya , Japan.
出版信息
Biosci Biotechnol Biochem. 2017 Jul;81(7):1456-1459. doi: 10.1080/09168451.2017.1314759. Epub 2017 Apr 13.
A Gram-positive bacterium Lentzea sp. 7887 hydroxylates a cyclosporine derivative FR901459 into AS1837812 (9-hydroxide), which is an important intermediate of candidate drugs that target the hepatitis C virus. We screened a UV-induced mutant, named M-1, which showed about 1.2-fold higher conversion yields, 2-fold higher substrate concentrations (3.69 mM), and 2.5-fold higher yield per unit volume than the wild-type strain.
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