Holtkamp W, Wuttke W, Nagel G A, Blossey H C
Abteilung Hämatologie/Onkologie, Zentrum Innere Medizin, Universitätskliniken Göttingen.
Onkologie. 1988 Apr;11(2):71-6. doi: 10.1159/000216491.
A receptor for lactogenic hormones (prolactin of several species and human growth hormone) was characterized in crude plasma membrane preparations of adult female rat liver. The binding of 125J-labeled prolactin to receptors was specific, saturable, reversible, and of high affinity (Kd = 0.23 x 10(-10)M). The maximum amount of prolactin specifically bound to liver of untreated female rats was 45 fmol/mg protein, whereas no specific prolactin binding was detected in plasma membrane preparations of adult male or immature rats. With this assay, a specific and reversible binding of ovine prolactin was detected in plasma membrane preparations of 25 human breast cancers, ranging from 2-29% of total activity. The estrogen-, progesterone- and androgen receptor content was determined in the breast cancer specimens using the dextran-coated charcoal method. Specimens with the highest specific prolactin binding showed the lowest steroid receptor concentrations, but no significant correlation between estrogen-, progesterone-, androgen-, and prolactin receptor content and other prognostic factors was observed in our series. We conclude, that steroid and prolactin receptors are expressed independently in human breast cancer.
在成年雌性大鼠肝脏的粗制质膜制剂中对催乳激素（几种动物的催乳素和人类生长激素）的受体进行了表征。125J标记的催乳素与受体的结合具有特异性、可饱和性、可逆性且亲和力高（Kd = 0.23×10⁻¹⁰M）。未处理的雌性大鼠肝脏中特异性结合的催乳素最大量为45 fmol/mg蛋白质，而在成年雄性或未成熟大鼠的质膜制剂中未检测到特异性催乳素结合。通过该检测方法，在25例人类乳腺癌的质膜制剂中检测到绵羊催乳素的特异性和可逆性结合，占总活性的2%至29%。使用葡聚糖包被活性炭法测定乳腺癌标本中的雌激素、孕激素和雄激素受体含量。特异性催乳素结合最高的标本显示类固醇受体浓度最低，但在我们的系列研究中未观察到雌激素、孕激素、雄激素和催乳素受体含量与其他预后因素之间存在显著相关性。我们得出结论，类固醇和催乳素受体在人类乳腺癌中独立表达。
