Department of Clinical Pharmacology, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, China.
State Key Laboratory of Quality Research in Chinese Medicine and School of Pharmacy, Macau University of Science and Technology, Macau.
Sci Rep. 2017 Apr 13;7:46278. doi: 10.1038/srep46278.
Hydrogen sulfide exists widely in mammalian tissues and plays a vital role in physiological and pathophysiological processes. However, striking differences with orders of magnitude were observed for the detected hydrogen sulfide concentrations in biological matrices among different measurements in literature, which lead to the uncertainty for examination the biological relevance of hydrogen sulfide. Here, we developed and validated a liquid chromatography- mass spectrometry (LC-MS/MS) method for the determination of hydrogen sulfide in various biological matrices by determination of a derivative of hydrogen sulfide and monobromobimane named sulfide dibimane (SDB). S-labeled SDB was synthesized and validated for using as an internal standard. This method has been successfully used to measure hydrogen sulfide levels in a broad range of biological matrices, such as blood, plasma, tissues, cells, and enzymes, across different species. Moreover, a novel mode that hydrogen sulfide could loosely and non-covalently bind to human serum protein (HSA) and hemoglobin (HB) was revealed by using the developed method.
硫化氢广泛存在于哺乳动物组织中，在生理和病理生理过程中发挥着重要作用。然而，文献中不同测量方法检测到的生物基质中硫化氢浓度存在显著差异，这导致硫化氢的生物学相关性的检验存在不确定性。在这里，我们通过测定硫化氢和单溴代丁二酰亚胺的衍生物，即硫化双丁二酰亚胺（SDB），开发并验证了一种用于各种生物基质中硫化氢测定的液相色谱-质谱（LC-MS/MS）方法。S 标记的 SDB 被合成并验证可作为内标使用。该方法已成功用于测量不同物种的血液、血浆、组织、细胞和酶等多种生物基质中的硫化氢水平。此外，通过使用所开发的方法，揭示了硫化氢可以松散非共价地结合到人血清蛋白（HSA）和血红蛋白（HB）的新方式。
