Gottofrey J, Borg K, Jasim S, Tjälve H
Department of Pharmacology and Toxicology, Swedish University of Agricultural Sciences, Uppsala, Sweden.
Pharmacol Toxicol. 1988 Jul;63(1):46-51. doi: 10.1111/j.1600-0773.1988.tb00908.x.
Brown trouts, Salmo trutta, were exposed to water containing 0.1 or 10 micrograms/l of 63Ni2+, alone or with potassium ethylxanthate or sodium diethyldithiocarbamate. After one and three weeks the accumulation and disposition of the 63Ni2+ in the fish were examined by liquid scintillation spectrometry and whole-body autoradiography. The sodium diethyldithiocarbamate was found to greatly enhance the uptake of 63Ni2+ in several tissues of the trouts. Potassium ethylxanthate was without effect. Diethyldithiocarbamate is known to form lipophilic complexes with metals, including nickel, and a facilitated penetration of the complexed nickel over the cellular membranes of the gills and other tissues is a likely mechanism underlying our results. The ethylxanthate is also able to form a lipophilic nickel-chelate, although of a lower lipophilicity than the nickel-diethyldithiocarbamate-complex. This variance in lipophilicity may explain why the disposition of the 63Ni2+ was affected by the diethyldithiocarbamate, but not by the ethylxanthate.
将褐鳟（Salmo trutta）暴露于含有0.1或10微克/升63Ni2+的水中，单独暴露或与乙基黄原酸钾或二乙基二硫代氨基甲酸钠共同暴露。在1周和3周后，通过液体闪烁光谱法和全身放射自显影检查63Ni2+在鱼体内的积累和分布情况。发现二乙基二硫代氨基甲酸钠能极大地增强63Ni2+在鳟鱼多个组织中的摄取。乙基黄原酸钾则没有作用。已知二乙基二硫代氨基甲酸盐能与包括镍在内的金属形成亲脂性络合物，络合镍更容易穿透鳃和其他组织的细胞膜可能是我们研究结果的潜在机制。乙基黄原酸盐也能够形成亲脂性镍螯合物，尽管其亲脂性低于镍 - 二乙基二硫代氨基甲酸盐络合物。这种亲脂性的差异可能解释了为什么63Ni2+的分布受到二乙基二硫代氨基甲酸盐的影响，而不受乙基黄原酸盐的影响。
