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白藜芦醇通过上调MFN2减少香烟烟雾提取物诱导的细胞凋亡。

Resveratrol reduces the apoptosis induced by cigarette smoke extract by upregulating MFN2.

作者信息

Song Chao, Luo Bailing, Gong Li

机构信息

Respiratory Department, Xiangya Hospital, Central South University, Changsha, Hunan Province,China.

出版信息

PLoS One. 2017 Apr 13;12(4):e0175009. doi: 10.1371/journal.pone.0175009. eCollection 2017.

DOI:10.1371/journal.pone.0175009
PMID:28406974
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5391199/
Abstract

PURPOSE

The aim of this study was to investigate the effect of resveratrol (RSV) on cigarette smoke extract (CSE)-induced cell apoptosis and mitochondrial dysfunction in vitro, as well as changes in the MFN2 expression level.

METHODS

Cultured human bronchial epithelial (HBE) cells were initially treated with CSE to induce apoptosis, followed by incubation either with or without RSV. Numerous techniques were used to evaluate the outcomes of the present study, including a cell counting kit-8 assay, real-time quantitative polymerase chain reaction (real-time qPCR), western blotting, JC-1 fluorescence, Hoechst 33342 staining, Annexin V-PI flow cytometry apoptosis analyses, and siRNA technology.

RESULTS

A 24 h incubation in 3.5% CSE induced apoptosis in HBE cells, and pretreatment of HBE cells with RSV (20 μM) significantly suppressed the CSE-induced apoptosis, prevented the CSE-induced decrease in MFN2 levels, suppressed BAX translocation to the mitochondria, and prevented mitochondrial membrane potential loss and cytochrome C release. However, following the transfection of MFN2 siRNA, the anti-apoptotic effects of RSV were significantly attenuated.

CONCLUSION

The results of the present study demonstrated that RSV may protect bronchial epithelial cells from CS-induced apoptosis in vitro by preventing mitochondrial dysfunction, and MFN2 may be associated with the anti-apoptotic functions of RSV in HBE cells.

摘要

目的

本研究旨在探讨白藜芦醇（RSV）对香烟烟雾提取物（CSE）诱导的体外细胞凋亡和线粒体功能障碍的影响，以及MFN2表达水平的变化。

方法

培养的人支气管上皮（HBE）细胞先用CSE处理以诱导凋亡，然后在有或无RSV的情况下孵育。使用了多种技术来评估本研究的结果，包括细胞计数试剂盒-8检测、实时定量聚合酶链反应（实时qPCR）、蛋白质免疫印迹、JC-1荧光、Hoechst 33342染色、膜联蛋白V-碘化丙啶流式细胞术凋亡分析和小干扰RNA技术。

结果

在3.5% CSE中孵育24小时可诱导HBE细胞凋亡，用RSV（20 μM）预处理HBE细胞可显著抑制CSE诱导的凋亡，防止CSE诱导的MFN2水平降低，抑制BAX向线粒体的转位，并防止线粒体膜电位丧失和细胞色素C释放。然而，转染MFN2小干扰RNA后，RSV的抗凋亡作用显著减弱。

结论

本研究结果表明，RSV可能通过防止线粒体功能障碍在体外保护支气管上皮细胞免受香烟烟雾诱导的凋亡，并且MFN2可能与RSV在HBE细胞中的抗凋亡功能有关。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2673/5391199/da6249eed9c4/pone.0175009.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2673/5391199/a8c66e6db7cf/pone.0175009.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2673/5391199/a244af045308/pone.0175009.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2673/5391199/094781521d6b/pone.0175009.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2673/5391199/e55efa78c93a/pone.0175009.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2673/5391199/da6249eed9c4/pone.0175009.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2673/5391199/a8c66e6db7cf/pone.0175009.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2673/5391199/a244af045308/pone.0175009.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2673/5391199/094781521d6b/pone.0175009.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2673/5391199/e55efa78c93a/pone.0175009.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2673/5391199/da6249eed9c4/pone.0175009.g005.jpg

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4
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4
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