Peeters Kelly, Zuliani Tea, Žigon Dušan, Milačič Radmila, Ščančar Janez
Department of Environmental Sciences, Jožef Stefan Institute, Jamova 39, 1000 Ljubljana, Slovenia.
Food Chem. 2017 Sep 1;230:327-335. doi: 10.1016/j.foodchem.2017.03.050. Epub 2017 Mar 10.
Nickel (Ni) is considered to be a potentially harmful element for humans. Its levels in foodstuffs are normally low (below 0.2mgkg), but sensitive individuals may develop allergy to Ni as a result of dietary consumption. Cocoa contains relatively high Ni concentrations (around 3mgkg). Ni bioavailability, its role in the flavour of food and its potential impact on human health depends primarily on its chemical species. However, there is a lack of information about Ni speciation in cocoa. In this work Ni species were separated on a weak convective interaction media diethylamine (CIM DEAE) monolithic chromatographic column and quantified by the post-column isotope dilution inductively coupled plasma mass spectrometry (ID-ICP-MS). The Ni binding ligands in the separated fractions were identified "off line" by quadrupole time-of-flight mass spectrometry (Q-TOF MS). Ni was found to be present in the cocoa infusions as Ni and Ni-gluconate and Ni-citrate complexes.
镍（Ni）被认为是一种对人体有潜在危害的元素。其在食品中的含量通常较低（低于0.2毫克/千克），但敏感个体可能因饮食摄入而对镍产生过敏反应。可可中镍的含量相对较高（约3毫克/千克）。镍的生物有效性、其在食物风味中的作用以及对人体健康的潜在影响主要取决于其化学形态。然而，目前缺乏关于可可中镍形态的信息。在这项研究中，镍的形态在弱对流相互作用介质二乙胺（CIM DEAE）整体色谱柱上进行分离，并通过柱后同位素稀释电感耦合等离子体质谱法（ID-ICP-MS）进行定量分析。通过四极杆飞行时间质谱法（Q-TOF MS）“离线”鉴定分离组分中的镍结合配体。研究发现，可可浸出液中的镍以镍、镍-葡萄糖酸盐和镍-柠檬酸盐络合物的形式存在。
