巴西不同成熟阶段高粱籽粒中细交链孢菌酮酸和高粱茎点霉的自然发生情况。
Natural occurrence of tenuazonic acid and Phoma sorghina in Brazilian sorghum grains at different maturity stages.
作者信息
Oliveira Rodrigo C, Goncalves Sarah S, Oliveira Mauricio S, Dilkin Paulo, Mallmann Carlos A, Freitas Rogerio S, Bianchi Priscilla, Correa Benedito
机构信息
Laboratory of Mycotoxins and Toxigenic Fungi, Institute of Biomedical Sciences, University of Sao Paulo, Sao Paulo, Brazil.
Center for Research in Medical Mycology, Federal University of Espirito Santo, Vitoria, Brazil.
出版信息
Food Chem. 2017 Sep 1;230:491-496. doi: 10.1016/j.foodchem.2017.03.079. Epub 2017 Mar 15.
A survey of 100 samples of sorghum grains was carried out to determine Phoma spp. and tenuazonic acid (TA) contamination using molecular tools and LC-MS/MS. Sorghum samples were obtained at the following four grain maturity stages: milk (S1), soft dough (S2), hard dough (S3), and physiological maturity (S4). The results revealed a good correlation between Phoma and TA occurrence during grain development. The samples showed Phoma contamination with frequencies ranging from 2.4% (S1) to 87.4% (S4), and the molecular identification revealed P. sorghina as the only Phoma specie isolated. Tenuazonic acid was found in sorghum grains at all maturity stages. In S2, S3 and S4, 100% of the samples showed TA contamination with levels ranging from 20 to 1234µg/kg. Low levels of TA were detected in 36% of the samples collected at S1 stage. This is the first report of tenuazonic acid in Brazilian sorghum grains.
为了利用分子工具和液相色谱-串联质谱法（LC-MS/MS）测定高粱籽粒中茎点霉属真菌和细交链孢菌酮酸（TA）的污染情况，对100份高粱籽粒样本进行了调查。高粱样本在以下四个籽粒成熟阶段采集：乳熟期（S1）、软面团期（S2）、硬面团期（S3）和生理成熟期（S4）。结果表明，在籽粒发育过程中，茎点霉属真菌的出现与细交链孢菌酮酸之间存在良好的相关性。样本中茎点霉属真菌的污染频率在2.4%（S1）至87.4%（S4）之间，分子鉴定表明分离出的唯一茎点霉属真菌种类为高粱茎点霉。在所有成熟阶段的高粱籽粒中均发现了细交链孢菌酮酸。在S2、S3和S4阶段，100%的样本显示出细交链孢菌酮酸污染，污染水平在20至1234µg/kg之间。在S1阶段采集的样本中，36%检测到低水平的细交链孢菌酮酸。这是巴西高粱籽粒中细交链孢菌酮酸的首次报道。
Zotero 插件