一种用于表征植物聚（ADP - 核糖）聚合酶（PARP）蛋白和抑制剂的酵母生长测定法。
A yeast growth assay to characterize plant poly(ADP-ribose) polymerase (PARP) proteins and inhibitors.
作者信息
Rissel Dagmar, Heym Peter Paul, Peiter Edgar
机构信息
Plant Nutrition Laboratory, Institute of Agricultural and Nutritional Sciences, Faculty of Natural Sciences III, Martin Luther University Halle-Wittenberg, 06099 Halle (Saale), Germany; Agrochemisches Institut Piesteritz e.V., 06886 Lutherstadt Wittenberg, Germany.
Sum of Squares, 04509 Delitzsch, Germany.
出版信息
Anal Biochem. 2017 Jun 15;527:20-23. doi: 10.1016/j.ab.2017.04.002. Epub 2017 Apr 10.
Poly(ADP-ribose) polymerases (PARPs) have been implicated in responses of plants to DNA damage and numerous stresses, whereby the mechanistic basis of the interference is often unclear. Therefore, the identification of specific inhibitors and potential interactors of plant PARPs is desirable. For this purpose, we established an assay based on heterologous expression of PARP genes from the model plant Arabidopsis thaliana in yeast. Expression of AtPARPs caused an inhibition of yeast growth to different extent, which was alleviated by inhibitors targeted at human PARPs. This assay provides a fast and simple means to identify target proteins and pharmacological inhibitors of AtPARP1.
聚（ADP - 核糖）聚合酶（PARP）与植物对DNA损伤和多种胁迫的反应有关，然而这种干扰的机制基础往往尚不清楚。因此，鉴定植物PARP的特异性抑制剂和潜在相互作用分子是很有必要的。为此，我们建立了一种基于在酵母中异源表达模式植物拟南芥PARP基因的检测方法。拟南芥PARP基因的表达对酵母生长产生了不同程度的抑制，而针对人类PARP的抑制剂可缓解这种抑制。该检测方法为鉴定AtPARP1的靶蛋白和药理抑制剂提供了一种快速简便的手段。
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