Vanderauwera Sandy, De Block Marc, Van de Steene Nancy, van de Cotte Brigitte, Metzlaff Michael, Van Breusegem Frank
Department of Plant Systems Biology, Flanders Institute for Biotechnology (VIB), Technologiepark 927, 9052 Ghent, Belgium.
Proc Natl Acad Sci U S A. 2007 Sep 18;104(38):15150-5. doi: 10.1073/pnas.0706668104. Epub 2007 Sep 6.
Transgenic plants with reduced poly(ADP-ribose) polymerase (PARP) levels have broad-spectrum stress-resistant phenotypes. Both Arabidopsis thaliana and oilseed rape (Brassica napus) lines overexpressing RNA interference-PARP constructs were more resistant to various abiotic stress treatments in laboratory and greenhouse experiments without negative effects on growth, development, and seed production. This outperforming stress tolerance was initially attributed solely to a maintained energy homeostasis due to reduced NAD(+) consumption. We show that in PARP2-deficient Arabidopsis plants, the observed abiotic stress resistance can also be explained by alterations in abscisic acid levels that facilitate the induction of a wide set of defense-related genes.
聚（ADP - 核糖）聚合酶（PARP）水平降低的转基因植物具有广谱抗逆表型。在实验室和温室实验中，过表达RNA干扰PARP构建体的拟南芥和油菜（甘蓝型油菜）品系对各种非生物胁迫处理更具抗性，且对生长、发育和种子生产没有负面影响。这种卓越的胁迫耐受性最初仅归因于由于NAD(+)消耗减少而维持的能量稳态。我们表明，在PARP2缺陷的拟南芥植物中，观察到的非生物胁迫抗性也可以通过脱落酸水平的改变来解释，这种改变促进了一系列与防御相关基因的诱导。
