Flynn W T, Saif L J, Moorhead P D
Ohio Agricultural Research and Development Center, Ohio State University, Wooster 44691.
Am J Vet Res. 1988 Jun;49(6):819-25.
Eighteen 4-day-old gnotobiotic pigs were orally inoculated with porcine enteric calicivirus-like virus (C strain). Seven additional gnotobiotic pigs served as noninoculated controls. Mild diarrhea developed in all inoculated pigs by postinoculation day (PID) 3 and persisted for 3 to 7 days. Severe diarrhea developed in 2 inoculated pigs between PID 4 and 5. Twelve inoculated and 7 control pigs were euthanatized over a 7-day period. Small intestinal mucosal smears were stained with a fluorescein-conjugated anti-porcine enteric calicivirus-like virus serum. Immunofluorescence was observed in villous epithelial cells (primarily in the duodenum or jejunum) of all inoculated pigs, except for 1 pig euthanatized at PID 7. Villus length was determined in histologic sections of the small intestinal specimens from control and inoculated pigs. Statistically significant (P less than 0.01) villus atrophy was found in the duodenum and/or jejunum of inoculated pigs at PID 3 to 7. These observations were confirmed by scanning electron microscopy, which revealed shortening, blunting, fusion, or absence of villi in the duodenum and jejunum of inoculated pigs at PID 3 to 7. Lesions were not seen in control pigs. Calicivirus-like particles were detected by immune electron microscopy in the large intestinal contents and feces of inoculated pigs from PID 1 to 7.
将18头4日龄无菌仔猪经口接种猪肠道杯状病毒样病毒（C株）。另外7头无菌仔猪作为未接种对照。所有接种仔猪在接种后第3天（PID 3）出现轻度腹泻，并持续3至7天。2头接种仔猪在PID 4至5天之间出现严重腹泻。在7天内对12头接种仔猪和7头对照仔猪实施安乐死。用荧光素标记的抗猪肠道杯状病毒样病毒血清对小肠黏膜涂片进行染色。除1头在PID 7实施安乐死的仔猪外，在所有接种仔猪的绒毛上皮细胞（主要在十二指肠或空肠）中观察到免疫荧光。测定对照仔猪和接种仔猪小肠标本组织切片中的绒毛长度。在PID 3至7时，接种仔猪的十二指肠和/或空肠中发现有统计学意义（P小于0.01）的绒毛萎缩。扫描电子显微镜证实了这些观察结果，其显示在PID 3至7时，接种仔猪的十二指肠和空肠中存在绒毛缩短、变钝、融合或缺失的情况。对照仔猪未见病变。在PID 1至7时，通过免疫电子显微镜在接种仔猪的大肠内容物和粪便中检测到杯状病毒样颗粒。
