Narita M, Fukusho A, Konno S, Shimizu Y
Natl Inst Anim Health Q (Tokyo). 1982 Summer;22(2):54-60.
Intestinal lesions were studied in 8 gnotobiotic piglets orally given porcine rotavirus by light microscopy, scanning electron microscopy and transmission electron microscopy. Five of these piglets were killed 6, 12, 18, 24 and 48 hours after inoculation, respectively. Of the other 3 piglets, one died 60 hours and two became moribund 96 and 114 hours after inoculation, respectively. All the infected piglets showed diarrhea between 18 and 24 hours after inoculation. Examination of the small intestine by light microscopy and scanning electron microscopy indicated the extensive degeneration and desquamation of epithelial cells with villi stripped and shortened. Villous degeneration, especially that in the jejunum and ileum, was very severe 24 hours after inoculation. Within 48 hours after inoculation, all the stripped villi were replaced by multiplication of regenerated cuboidal epithelial cells. Transmission electron microscopy revealed many virus particles in the epithelial cells of the small intestine 18 hours after inoculation.
通过光学显微镜、扫描电子显微镜和透射电子显微镜对8只经口接种猪轮状病毒的无菌仔猪的肠道病变进行了研究。其中5只仔猪分别在接种后6、12、18、24和48小时处死。另外3只仔猪中，1只在接种后60小时死亡，2只分别在接种后96和114小时濒死。所有感染仔猪在接种后18至24小时出现腹泻。光学显微镜和扫描电子显微镜检查小肠显示上皮细胞广泛变性和脱落，绒毛剥脱和缩短。接种后24小时，绒毛变性，尤其是空肠和回肠的绒毛变性非常严重。接种后48小时内，所有剥脱的绒毛被再生的立方上皮细胞增殖所取代。透射电子显微镜显示接种后18小时小肠上皮细胞中有许多病毒颗粒。
