Moura Tuane Carolina Ferreira, Amoras Ednelza da Silva Graça, Araújo Mauro Sérgio, Freitas Queiroz Maria Alice, Conde Simone Regina Souza da Silva, Demachki Sâmia, Martins-Feitosa Rosimar Neris, Machado Luiz Fernando Almeida, Cayres-Vallinoto Izaura Maria Vieira, Ishak Ricardo, Vallinoto Antonio Carlos Rosário
Laboratory of Virology, Institute of Biological Sciences, Universidade Federal do Pará (UFPA), Guamá, 66075-110 Belém, PA, Brazil.
João de Barros Barreto University Hospital, Federal University of Pará (Universidade Federal do Pará (UFPA)), Guamá, 66073-000 Belém, PA, Brazil.
Mediators Inflamm. 2017;2017:3718451. doi: 10.1155/2017/3718451. Epub 2017 Mar 12.
The present study investigated the frequencies of rs1800450 ( B, G>A), rs1800451 ( C, G>A), and rs5030737 ( D, C>T) polymorphisms in exon 1 of the gene among patients with chronic viral hepatitis. Blood samples from patients infected with hepatitis B virus (HBV; = 65), hepatitis C virus (HCV; = 92), and a noninfected control group ( = 300) were investigated. The presence of polymorphisms was detected using a real-time polymerase chain reaction to correlate with liver disease pathogenesis and fibrosis staging according to the Metavir classification. The genotypic and allelic frequencies showed no significant differences between the groups, but patients with active HBV and the wild genotype presented a positive correlation between increased transaminase and HBV DNA levels and the presence of mild to moderate fibrosis. Patients with HCV and the wild genotype presented mild inflammation and higher HCV RNA levels, although the same association was not observed for the fibrosis scores. The results suggest that the mutations in exon 1 of the gene do not contribute directly to the clinical and laboratory features of HCV and HBV infections, but further studies should be performed to confirm whether the wild genotype has indirect effect on disease progression.
本研究调查了慢性病毒性肝炎患者中该基因第1外显子的rs1800450（B，G>A）、rs1800451（C，G>A）和rs5030737（D，C>T）多态性的频率。对感染乙型肝炎病毒（HBV；n = 65）、丙型肝炎病毒（HCV；n = 92）的患者以及未感染的对照组（n = 300）的血样进行了研究。使用实时聚合酶链反应检测多态性的存在，并根据梅塔维分类法将其与肝病发病机制和纤维化分期相关联。基因型和等位基因频率在各组之间无显著差异，但活动性HBV患者和野生型基因型患者的转氨酶升高与HBV DNA水平以及轻度至中度纤维化的存在呈正相关。HCV患者和野生型基因型患者表现为轻度炎症和较高的HCV RNA水平，尽管在纤维化评分方面未观察到相同的关联。结果表明，该基因第1外显子的突变并不直接影响HCV和HBV感染的临床和实验室特征，但应进行进一步研究以确认野生型基因型是否对疾病进展有间接影响。
