Nilsson E, Palmblad J
Department of Medicine III, Karolinska Institutet, Stockholm, Sweden.
Biochem Pharmacol. 1988 Sep 1;37(17):3237-43. doi: 10.1016/0006-2952(88)90633-8.
Since the mechanisms for inhibitory effects of ethanol on polymorphonuclear (PMN) leukocyte function are largely unknown we studied secretory and aggregatory responses after in vitro treatment of PMN with ethanol. Oxidative metabolism (assessed by chemiluminescence, superoxide ion production and oxygen consumption) and aggregation were reduced dose-dependently in the presence of ethanol when elicited by stimuli binding to surface receptors or other structures (fMLP and ConA). Similar results were obtained with non-receptor-dependent stimuli, as NaF and the calcium ionophore A23187. In contrast, phorbol myristate acetate peak responses were not affected. FMLP induced changes in Fura-2AM fluorescence (as a probe for fast intracellular calcium concentration changes) were, however, not influenced by ethanol. Neither were membrane potential changes [assessed by di-O-C5(3) fluorescence]. Thus, it is suggested that ethanol may impair a step of the stimulus-response-coupling in PMNs distal to the initial intracellular calcium mobilization.
由于乙醇对多形核（PMN）白细胞功能的抑制作用机制在很大程度上尚不清楚，我们研究了乙醇体外处理PMN后的分泌和聚集反应。当由与表面受体或其他结构结合的刺激物（fMLP和刀豆球蛋白A）引发时，在乙醇存在下，氧化代谢（通过化学发光、超氧阴离子产生和氧消耗评估）和聚集呈剂量依赖性降低。用非受体依赖性刺激物（如氟化钠和钙离子载体A23187）也获得了类似结果。相比之下，佛波醇肉豆蔻酸酯乙酸盐的峰值反应不受影响。然而，fMLP诱导的Fura-2AM荧光变化（作为细胞内快速钙浓度变化的探针）不受乙醇影响。膜电位变化（通过二辛可尼酸（3）荧光评估）也不受影响。因此，提示乙醇可能损害PMN中初始细胞内钙动员远端的刺激-反应偶联步骤。
