• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

Cre-ERT2在体内的自发重组酶活性。

Spontaneous recombinase activity of Cre-ERT2 in vivo.

作者信息

Kristianto Jasmin, Johnson Michael G, Zastrow Ryley K, Radcliff Abigail B, Blank Robert D

机构信息

Department of Medicine, Medical College of Wisconsin, Milwaukee, WI, USA.

William S. Middleton Memorial Veterans Hospital, Madison, WI, USA.

出版信息

Transgenic Res. 2017 Jun;26(3):411-417. doi: 10.1007/s11248-017-0018-1. Epub 2017 Apr 13.

DOI:10.1007/s11248-017-0018-1
PMID:28409408
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9474299/
Abstract

Inducible Cre-ERT recombinase technology is widely used for gene targeting studies. The second generation of inducible Cre-ERT recombinase, hemizygous B6.129S-Tg(UBC-cre/ERT2)1Ejb/J (hereafter abbreviated as Cre-ERT2), a fusion of a mutated estrogen receptor and Cre recombinase, was engineered to be more efficient and specific than the original Cre-ERT. The putative mechanism of selective Cre-mediated recombination is Cre sequestration in the cytoplasm in the basal state with translocation to the nucleus only in the presence of tamoxifen. We utilized both a reporter mouse (B6.129 (Cg)-Gt(ROSA)26Sor /J) and endothelin converting enzyme-1 floxed transgenic mouse line to evaluate Cre-ERT2 activity. We observed spontaneous Cre activity in both settings. Unintended Cre activity is a confounding factor that has a potentially large impact on data interpretation. Thus, it is important to consider background Cre activity in experimental design.

摘要

诱导型Cre-ERT重组酶技术广泛应用于基因靶向研究。第二代诱导型Cre-ERT重组酶，半合子B6.129S-Tg(UBC-cre/ERT2)1Ejb/J（以下简称为Cre-ERT2），是一种突变雌激素受体与Cre重组酶的融合体，经设计后比原始的Cre-ERT更高效、更具特异性。选择性Cre介导重组的推测机制是，在基础状态下Cre隔离于细胞质中，仅在存在他莫昔芬时才易位至细胞核。我们利用报告基因小鼠（B6.129 (Cg)-Gt(ROSA)26Sor /J）和内皮素转换酶-1基因敲除转基因小鼠品系来评估Cre-ERT2的活性。我们在两种情况下均观察到了自发的Cre活性。意外的Cre活性是一个混杂因素，对数据解释可能有很大影响。因此，在实验设计中考虑背景Cre活性很重要。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6dd/9474299/dcedfb934144/nihms-1831649-f0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6dd/9474299/5fc0203ea22b/nihms-1831649-f0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6dd/9474299/0c61754552d2/nihms-1831649-f0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6dd/9474299/55fdabbf92b6/nihms-1831649-f0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6dd/9474299/dcedfb934144/nihms-1831649-f0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6dd/9474299/5fc0203ea22b/nihms-1831649-f0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6dd/9474299/0c61754552d2/nihms-1831649-f0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6dd/9474299/55fdabbf92b6/nihms-1831649-f0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6dd/9474299/dcedfb934144/nihms-1831649-f0004.jpg

相似文献

1
Spontaneous recombinase activity of Cre-ERT2 in vivo.Cre-ERT2在体内的自发重组酶活性。
Transgenic Res. 2017 Jun;26(3):411-417. doi: 10.1007/s11248-017-0018-1. Epub 2017 Apr 13.
2
Temporal control of gene deletion in sensory ganglia using a tamoxifen-inducible Advillin-Cre-ERT2 recombinase mouse.使用他莫昔芬诱导的 Advillin-Cre-ERT2 重组酶小鼠实现感觉神经节中基因缺失的时间控制。
Mol Pain. 2011 Dec 21;7:100. doi: 10.1186/1744-8069-7-100.
3
A novel inducible von Willebrand Factor Cre recombinase mouse strain to study microvascular endothelial cell-specific biological processes in vivo.一种新型可诱导的血管性血友病因子 Cre 重组酶小鼠品系，用于研究体内微血管内皮细胞特异性的生物学过程。
Vascul Pharmacol. 2024 Jun;155:107369. doi: 10.1016/j.vph.2024.107369. Epub 2024 Mar 29.
4
Tamoxifen-inducible NaV1.8-CreERT2 recombinase activity in nociceptive neurons of dorsal root ganglia.他莫昔芬诱导的背根神经节伤害性神经元中NaV1.8-CreERT2重组酶活性
Genesis. 2006 Aug;44(8):364-71. doi: 10.1002/dvg.20224.
5
Generation and characterization of transgenic mice expressing tamoxifen-inducible cre-fusion protein specifically in mouse liver.在小鼠肝脏中特异性表达他莫昔芬诱导型cre融合蛋白的转基因小鼠的构建与鉴定
World J Gastroenterol. 2003 Aug;9(8):1844-7. doi: 10.3748/wjg.v9.i8.1844.
6
Temporally-controlled site-specific mutagenesis in the basal layer of the epidermis: comparison of the recombinase activity of the tamoxifen-inducible Cre-ER(T) and Cre-ER(T2) recombinases.表皮基底层的时间控制位点特异性诱变：他莫昔芬诱导型Cre-ER(T)和Cre-ER(T2)重组酶重组活性的比较。
Nucleic Acids Res. 1999 Nov 15;27(22):4324-7. doi: 10.1093/nar/27.22.4324.
7
Controlling gene expression in the urothelium using transgenic mice with inducible bladder specific Cre-lox recombination.利用具有可诱导膀胱特异性Cre-lox重组的转基因小鼠控制尿路上皮中的基因表达。
J Urol. 2002 Dec;168(6):2641-4. doi: 10.1016/S0022-5347(05)64235-8.
8
Targeted Integration of Transgenes at the Mouse Gt(ROSA)26Sor Locus.转基因在小鼠Gt(ROSA)26Sor位点的靶向整合。
Methods Mol Biol. 2023;2631:299-323. doi: 10.1007/978-1-0716-2990-1_13.
9
A mouse model with tamoxifen-inducible thyrocyte-specific cre recombinase activity.一种具有他莫昔芬诱导型甲状腺细胞特异性cre重组酶活性的小鼠模型。
Genesis. 2014 Apr;52(4):333-40. doi: 10.1002/dvg.22740. Epub 2014 Jan 15.
10
VE-cadherin-CreERT2 transgenic mouse: a model for inducible recombination in the endothelium.血管内皮钙黏蛋白-CreERT2转基因小鼠：一种用于内皮细胞诱导性重组的模型。
Dev Dyn. 2006 Dec;235(12):3413-22. doi: 10.1002/dvdy.20982.

引用本文的文献

1
Ablation of macrophage transcriptional factor FoxO1 protects against ischemia-reperfusion injury-induced acute kidney injury.巨噬细胞转录因子FoxO1的缺失可预防缺血再灌注损伤诱导的急性肾损伤。
Acta Pharm Sin B. 2025 Jun;15(6):3107-3124. doi: 10.1016/j.apsb.2025.04.009. Epub 2025 Apr 10.
2
SWITCHER, a CRISPR-inducible floxed wild-type Cre regulating CRISPR activity.SWITCHER，一种CRISPR诱导的loxP侧翼野生型Cre，可调节CRISPR活性。
Commun Biol. 2025 Jul 2;8(1):982. doi: 10.1038/s42003-025-08427-8.
3
Endothelial cell-specific postnatal deletion of Nos3 preserves intraocular pressure homeostasis via macrophage recruitment and NOS2 upregulation.

本文引用的文献

1
Mitochondrial Dynamics is a Distinguishing Feature of Skeletal Muscle Fiber Types and Regulates Organellar Compartmentalization.线粒体动力学是骨骼肌纤维类型的一个显著特征，并调节细胞器的区室化。
Cell Metab. 2015 Dec 1;22(6):1033-44. doi: 10.1016/j.cmet.2015.09.027. Epub 2015 Oct 22.
2
Msx1CreERT2 knock-In allele: A useful tool to target embryonic and adult cardiac valves.Msx1CreERT2基因敲入等位基因：一种用于靶向胚胎和成年心脏瓣膜的有用工具。
Genesis. 2015 May;53(5):337-45. doi: 10.1002/dvg.22856.
3
Inducible cell labeling and lineage tracking during fracture repair.
出生后内皮细胞特异性缺失Nos3可通过巨噬细胞募集和NOS2上调维持眼内压稳态。
J Clin Invest. 2025 Feb 11;135(7):e183440. doi: 10.1172/JCI183440.
4
Generation of a genetically engineered porcine melanoma model featuring oncogenic control through conditional Cre recombination.通过条件性Cre重组实现致癌控制的基因工程猪黑色素瘤模型的构建。
Sci Rep. 2025 Jan 10;15(1):1616. doi: 10.1038/s41598-024-82554-w.
5
Development of a new flippase-dependent mouse model for red fluorescence-based isolation of KRAS oncogene-expressing tumor cells.用于基于红色荧光分离表达KRAS癌基因的肿瘤细胞的新型翻转酶依赖性小鼠模型的开发。
Transgenic Res. 2025 Jan 9;34(1):9. doi: 10.1007/s11248-024-00429-2.
6
CRISPR Knock-Ins in Organoids to Track Tumor Cell Subpopulations.类器官中的 CRISPR 基因敲入技术用于追踪肿瘤细胞亚群。
Methods Mol Biol. 2024;2811:137-154. doi: 10.1007/978-1-0716-3882-8_10.
7
Rational evolution for altering the ligand preference of estrogen receptor alpha.合理设计改变雌激素受体α配体偏好的进化。
Protein Sci. 2024 Apr;33(4):e4940. doi: 10.1002/pro.4940.
8
A Novel Cre Recombinase Mouse Strain for Cell-Specific Deletion of Floxed Genes in Ribbon Synapse-Forming Retinal Neurons.一种新型 Cre 重组酶小鼠品系，用于在形成 ribbon 突触的视网膜神经元中特异性删除 floxed 基因。
Int J Mol Sci. 2024 Feb 5;25(3):1916. doi: 10.3390/ijms25031916.
9
Comparative analyses of Netherton syndrome patients and Spink5 conditional knock-out mice uncover disease-relevant pathways.Netherton综合征患者与Spink5条件性基因敲除小鼠的比较分析揭示了疾病相关途径。
Commun Biol. 2024 Feb 5;7(1):152. doi: 10.1038/s42003-024-05780-y.
10
RuX: A Novel, Flexible, and Sensitive Mifepristone-Induced Transcriptional Regulation System.RuX：一种新型、灵活且灵敏的米非司酮诱导转录调控系统。
Int J Cell Biol. 2023 Oct 31;2023:7121512. doi: 10.1155/2023/7121512. eCollection 2023.
骨折修复过程中的诱导性细胞标记与谱系追踪。
Dev Growth Differ. 2015 Jan;57(1):10-23. doi: 10.1111/dgd.12184. Epub 2014 Nov 11.
4
Deletion of the developmentally essential gene ATR in adult mice leads to age-related phenotypes and stem cell loss.在成年小鼠中删除发育必需基因ATR会导致与年龄相关的表型和干细胞丢失。
Cell Stem Cell. 2007 Jun 7;1(1):113-126. doi: 10.1016/j.stem.2007.03.002.
5
A global double-fluorescent Cre reporter mouse.一种全球双荧光Cre报告基因小鼠。
Genesis. 2007 Sep;45(9):593-605. doi: 10.1002/dvg.20335.
6
Cross-contamination with tamoxifen induces transgene expression in non-exposed inducible transgenic mice.他莫昔芬的交叉污染会在未暴露的可诱导转基因小鼠中诱导转基因表达。
Genet Mol Res. 2004 Dec 30;3(4):456-62.
7
Tight control of transgene expression by lentivirus vectors containing second-generation tetracycline-responsive promoters.通过含有第二代四环素反应性启动子的慢病毒载体对转基因表达进行严格控制。
J Gene Med. 2005 Jun;7(6):803-17. doi: 10.1002/jgm.712.
8
Tamoxifen-inducible glia-specific Cre mice for somatic mutagenesis in oligodendrocytes and Schwann cells.用于少突胶质细胞和施万细胞体细胞诱变的他莫昔芬诱导型胶质细胞特异性Cre小鼠。
Mol Cell Neurosci. 2003 Apr;22(4):430-40. doi: 10.1016/s1044-7431(03)00029-0.
9
Evaluation of Tet-on system to avoid transgene down-regulation in ex vivo gene transfer to the CNS.评估Tet-on系统以避免在中枢神经系统的离体基因转移中出现转基因下调。
Gene Ther. 2002 Oct;9(19):1291-301. doi: 10.1038/sj.gt.3301778.
10
Germline transmission and tissue-specific expression of transgenes delivered by lentiviral vectors.慢病毒载体介导的转基因的种系传递和组织特异性表达。
Science. 2002 Feb 1;295(5556):868-72. doi: 10.1126/science.1067081. Epub 2002 Jan 10.