用于过氧化物酶体研究的原代人皮肤成纤维细胞转染

Transfection of Primary Human Skin Fibroblasts for Peroxisomal Studies.

作者信息

Koster Janet, Waterham Hans R

机构信息

Laboratory Genetic Metabolic Diseases, Department of Clinical Chemistry, Academic Medical Center, University of Amsterdam, Meibergdreef 9, 1105 AZ, Amsterdam, The Netherlands.

出版信息

Methods Mol Biol. 2017;1595:63-67. doi: 10.1007/978-1-4939-6937-1_7.

DOI:10.1007/978-1-4939-6937-1_7

PMID:28409452

Abstract

Functional studies with primary human skin fibroblasts from patients with a peroxisomal disorder often require efficient transfection with plasmids to correct the genetic defect or to express heterologous reporter proteins. Here, we describe a protocol we commonly use for efficient nonviral transfection of primary human skin fibroblasts.

摘要

对患有过氧化物酶体疾病患者的原代人皮肤成纤维细胞进行功能研究，通常需要用质粒进行高效转染，以纠正基因缺陷或表达异源报告蛋白。在此，我们描述一种我们常用的对原代人皮肤成纤维细胞进行高效非病毒转染的方案。