Bruno Valentina, Rizzacasa Barbara, Pietropolli Adalgisa, Capogna Maria Vittoria, Massoud Renato, Ticconi Carlo, Piccione Emilio, Cortese Claudio, Novelli Giuseppe, Amati Francesca
1 Academic Department of Biomedicine and Prevention, Section of Gynecology and Obstetrics, University of Rome Tor Vergata and Department of Surgery, Section of Gynecology and Obstetrics, Tor Vergata University Hospital , Rome, Italy .
2 Department of Surgery, Section of Gynecology and Obstetrics, Tor Vergata University Hospital, Rome , Italy .
Genet Test Mol Biomarkers. 2017 Jun;21(6):363-372. doi: 10.1089/gtmb.2016.0331. Epub 2017 Apr 14.
The aim of this study was to evaluate the expression of OLR1 and its alternative splicing isoform Loxin in unexplained recurrent miscarriage (uRM).
Sixty-three women of reproductive age were recruited and were divided into four groups: 18 pregnant and 23 non-pregnant women with uRM, and 12 pregnant and 10 non-pregnant women with physiological pregnancies. Complementary DNA derived from peripheral blood mononuclear cells (PBMCs) was analyzed by quantitative real-time PCR to evaluate the expression of OLR1 and Loxin. Oxidized low-density lipoproteins (ox-LDLs) were assayed from serum by a commercially available kit.
Pregnant uRM women presented with a higher, though not significant, OLR1/Loxin ratio and a higher ox-LDLs serum level (p ≤ 0.05) compared with pregnant control women. OLR1 and Loxin levels were significantly decreased in non-pregnant uRM women compared with the control (OLR1: 0.00018 vs. 0.00043, p ≤ 0.005; Loxin: 0.00018 vs. 0.00060, p ≤ 0.005, respectively). Loxin expression decreased by about two-thirds (p ≤ 0.005) in pregnant women compared with non-pregnant control women. A higher expression of OLR1 in pregnant women compared with non-pregnant women with uRM (p ≤ 0.05) was observed, but no variation in Loxin expression was observed.
The results of this study show an association of peripheral OLR1 and Loxin expression levels in uRM women, and they suggest the possible existence of an uncontrolled oxidative stress in these women in the first trimester of pregnancy.
本研究旨在评估氧化型低密度脂蛋白受体1（OLR1）及其可变剪接异构体洛辛（Loxin）在不明原因复发性流产（uRM）中的表达情况。
招募了63名育龄妇女，分为四组：18名怀孕和23名未怀孕的uRM妇女，以及12名怀孕和10名未怀孕的生理妊娠妇女。通过定量实时聚合酶链反应（qRT-PCR）分析外周血单个核细胞（PBMC）衍生的互补DNA，以评估OLR1和Loxin的表达。使用市售试剂盒从血清中检测氧化型低密度脂蛋白（ox-LDL）。
与怀孕对照组妇女相比，怀孕的uRM妇女的OLR1/Loxin比值较高，虽无统计学意义，但ox-LDL血清水平较高（p≤0.05）。与对照组相比，未怀孕的uRM妇女的OLR1和Loxin水平显著降低（OLR1：0.00018对0.00043，p≤0.005；Loxin：0.00018对0.00060，p≤0.005）。与未怀孕的对照妇女相比，怀孕妇女的Loxin表达降低了约三分之二（p≤0.005）。观察到怀孕妇女的OLR1表达高于未怀孕的uRM妇女（p≤0.05），但未观察到Loxin表达的差异。
本研究结果显示uRM妇女外周OLR1和Loxin表达水平之间存在关联，并提示这些妇女在妊娠早期可能存在不受控制的氧化应激。
