Moffatt Pierre, Geng Yeqing, Lamplugh Lisa, Nanci Antonio, Roughley Peter J
Research Center, Shriners Hospitals for Children - Canada, 1003 Boulevard Décarie, Montréal, H4A 0A9, QC, Canada.
Department of Human Genetics, Faculty of Medicine, McGill University, Montreal, QC, Canada.
J Negat Results Biomed. 2017 Apr 17;16(1):7. doi: 10.1186/s12952-017-0074-3.
In vitro studies suggest that the multiple functions of decorin are related to both its core protein and its dermatan sulfate chain. To determine the contribution of the dermatan sulfate chain to the functional properties of decorin in vivo, a mutant mouse whose decorin lacked a dermatan sulfate chain was generated.
Homozygous mice expressing only the decorin core protein developed and grew in a similar manner to wild type mice. In both embryonic and postnatal mice, all connective tissues studied, including cartilage, skin and cornea, appeared to be normal upon histological examination, and their collagen fibrils were of normal diameter and organization. In addition, abdominal skin wounds healed in an identical manner in the mutant and wild type mice.
The absence of a dermatan sulfate chain on decorin does not appear to overtly influence its functional properties in vivo.
体外研究表明，核心蛋白聚糖的多种功能与其核心蛋白及其硫酸皮肤素链均有关。为了确定硫酸皮肤素链对体内核心蛋白聚糖功能特性的作用，制备了一种核心蛋白聚糖缺乏硫酸皮肤素链的突变小鼠。
仅表达核心蛋白聚糖核心蛋白的纯合小鼠的发育和生长方式与野生型小鼠相似。在胚胎期和出生后的小鼠中，所有研究的结缔组织，包括软骨、皮肤和角膜，经组织学检查似乎均正常，其胶原纤维直径和组织结构也正常。此外，突变小鼠和野生型小鼠腹部皮肤伤口的愈合方式相同。
核心蛋白聚糖上缺乏硫酸皮肤素链似乎并未明显影响其在体内的功能特性。
