Bae In-Suk, Kim Choong-Hyun, Kim Jae-Min, Cheong Jin-Hwan, Ryu Je-Il, Han Myung-Hoon
Department of Neurosurgery, Hanyang University Guri Hospital, Guri-si, Gyeonggi-do 11923, Republic of Korea.
Biomed Rep. 2017 Apr;6(4):396-400. doi: 10.3892/br.2017.861. Epub 2017 Feb 17.
Apoptosis, whose mechanism remains unclear, is regulated by multiple factors. B-cell lymphoma 2 (Bcl-2) is a well-known anti-apoptotic mediator. Survivin is also a recently recognized novel family inhibitor of apoptosis protein, which inhibits apoptosis via a pathway distinct from Bcl-2 family members. Survivin and Bcl-2 are expressed in various types of human cancer. In the present study, survivin and Bcl-2 expression were characterized in glial cell tumors, and the correlation with pathological malignancy and anti-apoptotic properties were investigated. Fifty-eight patients who had undergone surgical resection for glial cell tumors were evaluated. The pathological types of glial cell tumors were categorized according to the World Health Organization classification. Survivin and Bcl-2 expression levels were investigated by western blot analysis, and apoptosis was detected by DNA fragmentation analysis. The anti-apoptotic rate of glial cell tumors was calculated in tumor samples according to the expression of survivin and Bcl-2 or co-expression. Survivin was characterized in 60.3%, and Bcl-2 was expressed in 43.1% of glioma samples. Co-expression of survivin and Bcl-2 was observed in 25.9% of the tumor specimens. Survivin expression in astrocytic tumors was identified to be significantly associated with the pathological grade (P<0.05); however, Bcl-2 was not (P>0.05). Anti-apoptotic rate of glial cell tumors were detected in 91.4, 92.0 and 100% of patients exhibiting survivin, Bcl-2 or co-expression, respectively. However, the difference in anti-apoptotic frequency between the three groups was not identified to be statistically significant (P>0.05). The present study suggests that survivin expression is correlated with pathological grades of gliomas. In addition, the expression of survivin or Bcl-2 exerts potent anti-apoptotic properties in gliomas. Thus, survivin or Bcl-2 may serve as potential targets for inducing the apoptosis of gliomas.
凋亡受多种因素调控，但其机制尚不清楚。B细胞淋巴瘤2（Bcl-2）是一种著名的抗凋亡介质。生存素也是一种最近被认可的新型凋亡抑制蛋白家族成员，它通过一条不同于Bcl-2家族成员的途径抑制凋亡。生存素和Bcl-2在多种人类癌症中均有表达。在本研究中，对胶质细胞瘤中生存素和Bcl-2的表达进行了表征，并研究了其与病理恶性程度和抗凋亡特性的相关性。对58例接受胶质细胞瘤手术切除的患者进行了评估。胶质细胞瘤的病理类型根据世界卫生组织分类进行划分。通过蛋白质免疫印迹分析研究生存素和Bcl-2的表达水平，并通过DNA片段化分析检测凋亡情况。根据生存素和Bcl-2的表达或共表达情况，计算肿瘤样本中胶质细胞瘤的抗凋亡率。60.3%的胶质瘤样本中有生存素表达，43.1%的样本中有Bcl-2表达。25.9%的肿瘤标本中观察到生存素和Bcl-2共表达。星形细胞瘤中生存素的表达与病理分级显著相关（P<0.05）；然而，Bcl-2则不然（P>0.05）。分别在91.4%、92.0%和100%表现出生存素、Bcl-2或共表达的患者中检测到胶质细胞瘤的抗凋亡率。然而，三组之间抗凋亡频率的差异未被确定具有统计学意义（P>0.05）。本研究表明，生存素的表达与胶质瘤的病理分级相关。此外，生存素或Bcl-2的表达在胶质瘤中发挥强大的抗凋亡特性。因此，生存素或Bcl-2可能作为诱导胶质瘤凋亡的潜在靶点。
