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通过在人肝癌细胞系中瞬时表达鸭乙型肝炎病毒（DHBV）DNA产生的DHBV颗粒在体外具有传染性。

Duck hepatitis B virus (DHBV) particles produced by transient expression of DHBV DNA in a human hepatoma cell line are infectious in vitro.

作者信息

Pugh J C, Yaginuma K, Koike K, Summers J

机构信息

Fox Chase Cancer Center, Institute for Cancer Research, Philadelphia, Pennsylvania 19111.

出版信息

J Virol. 1988 Sep;62(9):3513-6. doi: 10.1128/JVI.62.9.3513-3516.1988.

DOI:10.1128/JVI.62.9.3513-3516.1988
PMID:2841501
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC253480/
Abstract

Transfection of the human hepatocellular carcinoma cell line HuH7 with a plasmid containing a tandem copy of the duck hepatitis B virus DNA sequence resulted in transient replication of the virus. Viral particles secreted by transfected HuH7 cells exhibited physical properties similar to those of serum-derived duck hepatitis B virus and were infectious in primary duck hepatocyte cultures.

摘要

用人肝癌细胞系HuH7转染含有串联拷贝鸭乙型肝炎病毒DNA序列的质粒，导致病毒短暂复制。转染的HuH7细胞分泌的病毒颗粒表现出与血清来源的鸭乙型肝炎病毒相似的物理特性，并且在原代鸭肝细胞培养物中具有感染性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7321/253480/0365d29be76b/jvirol00088-0454-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7321/253480/8911bac4be4a/jvirol00088-0452-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7321/253480/3d35720a110e/jvirol00088-0452-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7321/253480/4a67de2a2a3b/jvirol00088-0453-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7321/253480/0365d29be76b/jvirol00088-0454-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7321/253480/8911bac4be4a/jvirol00088-0452-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7321/253480/3d35720a110e/jvirol00088-0452-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7321/253480/4a67de2a2a3b/jvirol00088-0453-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7321/253480/0365d29be76b/jvirol00088-0454-a.jpg

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本文引用的文献

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Virus of Pekin ducks with structural and biological relatedness to human hepatitis B virus.与人类乙型肝炎病毒具有结构和生物学相关性的北京鸭病毒。
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Growth of human hepatoma cells lines with differentiated functions in chemically defined medium.
乙型肝炎病毒通过细胞内循环生成共价闭合环状 DNA 的方式受到病毒特异性调节。
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Effect of oxymatrine on the replication cycle of hepatitis B virus in vitro.氧化苦参碱体外抗乙型肝炎病毒复制作用的研究。
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Viral and cellular determinants involved in hepadnaviral entry.参与嗜肝DNA病毒进入的病毒和细胞决定因素。
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Enhancement of hepatitis B virus replication by the regulatory X protein in vitro and in vivo.调节性X蛋白在体外和体内增强乙型肝炎病毒复制
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N-terminal and C-terminal cytosine deaminase domain of APOBEC3G inhibit hepatitis B virus replication.载脂蛋白B mRNA编辑酶催化多肽样蛋白3G（APOBEC3G）的N端和C端胞嘧啶脱氨酶结构域抑制乙型肝炎病毒复制。
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Inhibition of hepatitis B virus replication by APOBEC3G in vitro and in vivo.载脂蛋白B mRNA编辑酶催化多肽样蛋白3G在体外和体内对乙型肝炎病毒复制的抑制作用。
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A new technique for the assay of infectivity of human adenovirus 5 DNA.一种检测人腺病毒5型DNA感染性的新技术。
Virology. 1973 Apr;52(2):456-67. doi: 10.1016/0042-6822(73)90341-3.
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The duck hepatitis B virus pre-C region encodes a signal sequence which is essential for synthesis and secretion of processed core proteins but not for virus formation.鸭乙型肝炎病毒前C区编码一个信号序列，该序列对于加工后的核心蛋白的合成和分泌至关重要，但对于病毒形成并非必需。
J Virol. 1987 Dec;61(12):3701-9. doi: 10.1128/JVI.61.12.3701-3709.1987.
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In vitro experimental infection of primary duck hepatocyte cultures with duck hepatitis B virus.用鸭乙型肝炎病毒对原代鸭肝细胞培养物进行体外实验性感染。
J Virol. 1986 Apr;58(1):17-25. doi: 10.1128/JVI.58.1.17-25.1986.
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Production of hepatitis B virus in vitro by transient expression of cloned HBV DNA in a hepatoma cell line.通过在肝癌细胞系中瞬时表达克隆的乙肝病毒（HBV）DNA在体外产生乙肝病毒
EMBO J. 1987 Mar;6(3):675-80. doi: 10.1002/j.1460-2075.1987.tb04807.x.
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Proc Natl Acad Sci U S A. 1987 May;84(9):2678-82. doi: 10.1073/pnas.84.9.2678.