Summers J, Mason W S
Cell. 1982 Jun;29(2):403-15. doi: 10.1016/0092-8674(82)90157-x.
Duck hepatitis B virus, a DNA virus closely related to human hepatitis B virus, was studied in infected duck liver. Subviral particles resembling the viral nucleocapsid cores were isolated from persistently infected liver and shown to have a DNA polymerase activity that utilizes an endogenous template and synthesizes both plus- and minus-strand viral DNA. Synthesis of the viral minus-strand DNA utilized an RNA template that was degraded as it was copied. Viral plus-strand synthesis occurred on the completed minus-strand DNA. A pathway for the replication of the DNA genome of hepatitis B-like viruses by reverse transcription of an RNA intermediate is proposed.
鸭乙型肝炎病毒是一种与人类乙型肝炎病毒密切相关的DNA病毒，已在受感染的鸭肝脏中进行了研究。从持续感染的肝脏中分离出了类似于病毒核衣壳核心的亚病毒颗粒，并显示其具有DNA聚合酶活性，该活性利用内源性模板合成正链和负链病毒DNA。病毒负链DNA的合成利用了一种RNA模板，该模板在被复制时会被降解。病毒正链合成发生在完整的负链DNA上。本文提出了一种通过RNA中间体的逆转录来复制乙型肝炎样病毒DNA基因组的途径。
