Roivainen M, Hovi T
Department of Virology, National Public Health Institute, Helsinki, Finland.
J Virol. 1988 Sep;62(9):3536-9. doi: 10.1128/JVI.62.9.3536-3539.1988.
We have exposed 22 independent type 2 poliovirus isolates to human intestinal fluid and purified trypsin. In all cases the virus retained its infectivity, while polyacrylamide gel electrophoresis of viral proteins showed disappearance of the VP1 bands. Concomitantly, the viruses became resistant to antigenic site 1-specific monoclonal antibodies, indicating that the cleavage took place at the antigenic site 1. Sera from persons immunized solely with the inactivated poliovirus vaccine (IPV) neutralized intact type 2 polioviruses more readily than the corresponding trypsin-cleaved virus preparations. The ratio between the neutralization indices for the intact and trypsin-cleaved type 2 polioviruses was not significantly changed by a dose of trivalent oral poliovirus vaccine given to children previously immunized with IPV. These results indicate that while the antigenic site 1 of type 2 poliovirus is immunogenic in humans when IPV is used, the relative role of this antigenic site in human immunity appears to be less critical than that in the case of type 3 polioviruses. Before we obtained these results, only antigenic site 1 had been shown to be immunogenic in type 2 polioviruses.
我们将22株独立的2型脊髓灰质炎病毒分离株暴露于人体肠液和纯化的胰蛋白酶中。在所有情况下，病毒都保持了其感染性，而病毒蛋白的聚丙烯酰胺凝胶电泳显示VP1条带消失。同时，这些病毒对抗抗原位点1特异性单克隆抗体产生了抗性，表明裂解发生在抗原位点1。仅接种灭活脊髓灰质炎病毒疫苗（IPV）的人的血清比相应的经胰蛋白酶裂解的病毒制剂更容易中和完整的2型脊髓灰质炎病毒。先前接种过IPV的儿童服用一剂三价口服脊髓灰质炎病毒疫苗后，完整和经胰蛋白酶裂解的2型脊髓灰质炎病毒的中和指数之比没有显著变化。这些结果表明，虽然使用IPV时2型脊髓灰质炎病毒的抗原位点1在人类中具有免疫原性，但该抗原位点在人类免疫中的相对作用似乎不如3型脊髓灰质炎病毒那样关键。在我们获得这些结果之前，只有抗原位点1被证明在2型脊髓灰质炎病毒中具有免疫原性。
