Rajabi Hossein, Noroozifar Meissam
Analytical Research Laboratory, Department of Chemistry, University of Sistan and Baluchestan, Zahedan, P.O. Box 98155-674, Iran.
Analytical Research Laboratory, Department of Chemistry, University of Sistan and Baluchestan, Zahedan, P.O. Box 98155-674, Iran.
Mater Sci Eng C Mater Biol Appl. 2017 Jun 1;75:791-797. doi: 10.1016/j.msec.2017.02.133. Epub 2017 Feb 27.
Uric acid (UA) and folic acid (FA) are compounds of biomedical interest. In humans, about 70% of daily uric acid disposal occurs via the kidneys, and in 5-25% of humans, impaired renal (kidney) excretion leads to hyperuricemia. Folate is another form folic acid of which is known as, is one of the B vitamins. It is used as a supplement by women to prevent neural tube defects developing during pregnancy. Polyortho-methoxyaniline nanostructures (POMANS) was synthesized with a new two phase (organic-water) synthesis method. The POMANS was characterized using transmission electron microscopy (TEM) and Fourier transform IR (FTIR). This polymer was used to construct a modified multi-wall carbon nanotube, graphite paste electrode (POMANS-MWCNT/GPE). Linear sweep voltammograms (LSV), cyclic voltammetry (CV) and chronoamperometry were used to investigate the suitability of polyortho-methoxyaniline with multi-wall carbon nanotubes paste electrode as a modifier for the electrocatalytic oxidation of UA and FA in aqueous solutions with various pHs. The results showed that POMANS-MWCNT/GPE had high anodic peak currents for the electrooxidation of UA and FA in pH6.0.Under the optimized conditions, The catalytic peak currents obtained, was linearly dependent on the UA and FA concentrations in the range of 0.6-52 and 0.5-68μM with two segments and the detection limits 0.157 and 0.113μM for UA and FA were, respectively. Finally, the proposed method was also examined as a sensitive, simple and inexpensive electrochemical sensor for the simultaneous determination of UA and FA in real samples such as urine and serum.
尿酸（UA）和叶酸（FA）是具有生物医学研究价值的化合物。在人类中，每日尿酸排泄量的约70%通过肾脏进行，而在5% - 25%的人群中，肾脏排泄功能受损会导致高尿酸血症。叶酸是叶酸的另一种形式，它是B族维生素之一。女性用它作为补充剂以预防孕期神经管缺陷。采用一种新的两相（有机 - 水）合成方法合成了聚邻甲氧基苯胺纳米结构（POMANS）。利用透射电子显微镜（TEM）和傅里叶变换红外光谱（FTIR）对POMANS进行了表征。该聚合物用于构建修饰的多壁碳纳米管石墨糊电极（POMANS - MWCNT/GPE）。采用线性扫描伏安法（LSV）、循环伏安法（CV）和计时电流法研究了聚邻甲氧基苯胺与多壁碳纳米管糊电极作为修饰剂对不同pH值水溶液中UA和FA进行电催化氧化的适用性。结果表明，POMANS - MWCNT/GPE在pH6.0时对UA和FA的电氧化具有高阳极峰电流。在优化条件下，获得的催化峰电流在0.6 - 52μM和0.5 - 68μM范围内与UA和FA浓度呈两段线性相关，UA和FA的检测限分别为0.157和0.113μM。最后，该方法还被用作一种灵敏、简单且廉价的电化学传感器，用于同时测定尿液和血清等实际样品中的UA和FA。
