Kuşat Kevser, Şanli Serdar, Timur Suna, Akgöl Sinan
Department of Chemistry, Faculty of Science, Dokuz Eylül University, İzmir, Turkey.
Department of Chemistry, Faculty of Science & Arts, Ordu University, Ordu, Turkey.
Turk J Chem. 2022 Apr 11;46(4):1210-1225. doi: 10.55730/1300-0527.3428. eCollection 2022.
Folic acid, which provides the transfer of single carbon atoms in synthesis reactions and metabolic cycles in metabolism, is very important for metabolism. Folic acid also plays an important role in nucleotide synthesis and methylation reactions. There are many disorders caused by defective folic acid metabolism and lack of folic acid. Today, innovative, cost-effective methods are needed to develop folic acid determination methods. The main objective of this study is the development of surface-printed carbon electrodes (SPCE) modified with folic acid imprinted nanostructures (FA-Imp-poly(MPTS-rGO-co-NAT), which will be used for the first time for folic acid determination in commercially human blood serum. For this purpose, the synthesis of nanostructures has been carried out and characterized by FTIR, SEM-EDS, and AFM. Then, a new chemically modified nanosensor was fabricated for the determination of folic acid using folic acid imprinted nanostructures. Differential pulse voltammetry (DPV) and circular voltammetry (CV) methods were used as electrochemical methods in the FA-imprinted-nanosensor studies. Measurements in differential pulse voltammetry were performed at an application speed of 0.005 volts per second in the potential range of -0.4 to 0.6 volts. As a result of the circular voltammetric method, an idea about the surface was obtained with the voltammograms obtained. The detection limit (LOD) of the developed FA-imprinted-nanosensor was 7.54 ng/mL and the determination limit (LOQ) was 25.14 ng/mL. FA analytical (10 and 20 ng/mL) was added to commercial synthetic serum samples by the standard adding method and RSD values of 0.092% and 0.734% were found in the DPV technique and measurements respectively. This manuscript demonstrated a novel, simple, selective, and rapid FA-imprinted-nanosensor for determining the FA in the biological samples.
叶酸在新陈代谢的合成反应和代谢循环中负责单碳原子的转移，对新陈代谢非常重要。叶酸在核苷酸合成和甲基化反应中也起着重要作用。叶酸代谢缺陷和叶酸缺乏会引发多种疾病。如今，需要创新且经济高效的方法来开发叶酸测定方法。本研究的主要目标是开发一种用叶酸印迹纳米结构（FA-Imp-poly(MPTS-rGO-co-NAT)）修饰的表面印刷碳电极（SPCE），该电极将首次用于商业人血清中叶酸的测定。为此，已进行了纳米结构的合成，并通过傅里叶变换红外光谱（FTIR）、扫描电子显微镜-能谱仪（SEM-EDS）和原子力显微镜（AFM）对其进行了表征。然后，利用叶酸印迹纳米结构制备了一种用于测定叶酸的新型化学修饰纳米传感器。在FA印迹纳米传感器研究中，采用差分脉冲伏安法（DPV）和循环伏安法（CV）作为电化学方法。差分脉冲伏安法的测量在-0.4至0.6伏的电位范围内以每秒0.005伏的施加速度进行。通过循环伏安法，从获得的伏安图中了解了表面情况。所开发的FA印迹纳米传感器的检测限（LOD）为7.54 ng/mL，定量限（LOQ）为25.14 ng/mL。通过标准加入法将FA分析物（10和20 ng/mL）添加到商业合成血清样品中，在DPV技术和测量中分别发现相对标准偏差（RSD）值为0.092%和0.734%。本论文展示了一种用于测定生物样品中FA的新型、简单、选择性好且快速的FA印迹纳米传感器。
