Ge Jingqiu, Hu Yijie, Wang Hongxia, Huang Yuanshe, Zhang Peng, Liao Zhihua, Chen Min
College of Pharmaceutical Sciences, Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, Southwest University, Chongqing, PR China.
National Key Laboratory of Plant Molecular Genetics, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Science, Shanghai, PR China.
J Sci Food Agric. 2017 Nov;97(14):4995-5003. doi: 10.1002/jsfa.8379. Epub 2017 Jun 5.
Anthocyanins in purple-fleshed sweet potato (PSP) are beneficial to human health. The leaf color (Lc) gene is a transcription factor involved in regulating anthocyanin biosynthesis. The anthocyanin profiles of wild-type PSP of Ayamurasaki and its three Lc-transgenic lines were investigated by high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). In vitro antioxidant activities of wild-type and Lc-transgenic lines, including reducing power activity, DPPH radical scavenging activity, hydroxyl radical scavenging activity, linoleic acid autoxidation inhibition activity, ABTS free radical scavenging activity and oxygen radical absorbance capacity activity, were measured.
The results showed that the total anthocyanin contents increased 1.5-1.9 times in three transgenic lines compared with that in wild-type PSP. Seventeen anthocyanins were found in wild-type PSP, while 19 in Lc-transgenic lines including cyanidin-based, peonidin-based and pelargonidin-based anthocyanins. Three pelargonidin-based anthocyanins were detected in three Lc-transgenic lines. Among them, the relative contents of cyanidin-based and pelargonidin-based anthocyanins increased 1.9-2.0 and 3.4-4.5 times respectively, while peonidin-based anthocyanins decreased 1.8-1.9 times in Lc-transgenic lines, compared with wild-type PSP. PSP from wild-type Ayamurasaki and three Lc-transgenic lines exhibited potent antioxidant activities, whereas there was no distinct difference among them.
The transgene Lc significantly increased the content of total anthocyanins and remarkably changed the anthocyanin profiles in Ayamurasaki. Such novel and high content of anthocyanins obtained in the Lc-transgenic lines with potent antioxidant activities may provide unique functional products with potential helpful for human health. © 2017 Society of Chemical Industry.
紫肉甘薯中的花青素对人体健康有益。叶色（Lc）基因是参与调控花青素生物合成的转录因子。采用高效液相色谱 - 串联质谱法（HPLC-MS/MS）研究了秋紫甘薯野生型及其三个Lc转基因系的花青素谱。测定了野生型和Lc转基因系的体外抗氧化活性，包括还原力活性、DPPH自由基清除活性、羟基自由基清除活性、亚油酸自氧化抑制活性、ABTS自由基清除活性和氧自由基吸收能力活性。
结果表明，三个转基因系的总花青素含量比野生型紫肉甘薯增加了1.5 - 1.9倍。在野生型紫肉甘薯中发现了17种花青素，而在Lc转基因系中发现了19种，包括矢车菊素类、芍药素类和天竺葵素类花青素。在三个Lc转基因系中检测到三种天竺葵素类花青素。其中，与野生型紫肉甘薯相比，Lc转基因系中矢车菊素类和天竺葵素类花青素的相对含量分别增加了1.9 - 2.0倍和3.4 - 4.5倍，而芍药素类花青素减少了1.8 - 1.9倍。秋紫甘薯野生型和三个Lc转基因系均表现出较强的抗氧化活性，但它们之间没有明显差异。
转基因Lc显著增加了秋紫甘薯的总花青素含量，并显著改变了花青素谱。在具有强抗氧化活性的Lc转基因系中获得的这种新颖且高含量的花青素可能提供对人类健康有潜在帮助的独特功能产品。© 2017化学工业协会。
