Kettle A J, Sangster D F, Gebicki J M, Winterbourn C C
Department of Pathology, Christchurch School of Medicine, Christchurch Hospital, New Zealand.
Biochim Biophys Acta. 1988 Aug 31;956(1):58-62. doi: 10.1016/0167-4838(88)90297-x.
Using pulse radiolysis, the rate constant for the reaction of ferric myeloperoxidase with O2- to give compound III was measured at pH 7.8, and values of 2.1.10(6) M-1.s-1 for equine ferric myeloperoxidase and 1.1.10(6) M-1.s-1 for human ferric myeloperoxidase were obtained. Under the same conditions, the rate constant for the reaction of human ferric myeloperoxidase with H2O2 to give compound I was 3.1.10(7) M-1.s-1. Our results indicate that although the reaction of ferric myeloperoxidase with O2- is an order of magnitude slower than with H2O2, the former reaction is sufficiently rapid to influence myeloperoxidase-dependent production of hypochlorous acid by stimulated neutrophils.
利用脉冲辐解技术，在pH 7.8条件下测定了高铁髓过氧化物酶与超氧阴离子反应生成化合物III的速率常数，得到马高铁髓过氧化物酶的值为2.1×10⁶ M⁻¹·s⁻¹，人高铁髓过氧化物酶的值为1.1×10⁶ M⁻¹·s⁻¹。在相同条件下，人高铁髓过氧化物酶与过氧化氢反应生成化合物I的速率常数为3.1×10⁷ M⁻¹·s⁻¹。我们的结果表明，尽管高铁髓过氧化物酶与超氧阴离子的反应比与过氧化氢的反应慢一个数量级，但前一个反应足够快，足以影响受刺激的中性粒细胞中髓过氧化物酶依赖的次氯酸生成。
