Oda Hitomi, Mori Akihiro, Hirowatari Yuji, Takoura Toshie, Manita Daisuke, Takahashi Tomoya, Shono Saori, Onozawa Eri, Mizutani Hisashi, Miki Yohei, Itabashi Yukiko, Sako Toshinori
School of Veterinary Nursing & Technology, Nippon Veterinary and Life Science University, Tokyo, Japan.
School of Veterinary Nursing & Technology, Nippon Veterinary and Life Science University, Tokyo, Japan.
Res Vet Sci. 2017 Oct;114:163-169. doi: 10.1016/j.rvsc.2017.04.004. Epub 2017 Apr 8.
Anion-exchange (AEX)-high-performance liquid chromatography (HPLC) for measurement of cholesterol can be used to separate serum lipoproteins (high-density lipoprotein (HDL); low-density lipoprotein (LDL); intermediate-density lipoprotein (IDL); very-low-density lipoprotein (VLDL)) in humans. However, AEX-HPLC has not been applied in veterinary practice. We had three objectives: (i) the validation of AEX-HPLC methods including the correlation of serum cholesterol concentration in lipoprotein fraction measured by AEX-HPLC and gel permeation-HPLC (GP-HPLC) in healthy dogs and those with hypercholesterolemia was investigated; (ii) the reference intervals of lipoprotein fractions measured by AEX-HPLC from healthy dogs (n=40) was established; (iii) lipoprotein fractions from the serum of healthy dogs (n=12) and dogs with hypercholesterolemia (n=23) were compared. Analytic reproducibility and precision of AEX-HPLC were acceptable. Positive correlation between serum concentrations of total cholesterol (Total-Chol), HDL cholesterol (HDL-Chol), LDL cholesterol (LDL-Chol)+IDL cholesterol (IDL-Chol), and VLDL cholesterol (VLDL-Chol) was noted for AEX-HPLC and GP-HPLC in healthy dogs and dogs with hypercholesterolemia. Reference intervals measured by AEX-HPLC for serum concentrations of Total-Chol, HDL-Chol, and LDL-Chol were determined to be 2.97-9.32, 2.79-6.57, 0.16-3.28mmol/L (2.5-97.5% interval), respectively. Furthermore, there was significant difference in lipoprotein profiles between healthy and dogs with hypercholesterolemia. These results suggest that AEX-HPLC can be used to evaluate lipoprotein profiles in dogs and could be a new useful indicator of hyperlipidemia in dogs.
用于测量胆固醇的阴离子交换（AEX）-高效液相色谱（HPLC）可用于分离人类血清脂蛋白（高密度脂蛋白（HDL）；低密度脂蛋白（LDL）；中间密度脂蛋白（IDL）；极低密度脂蛋白（VLDL））。然而，AEX-HPLC尚未应用于兽医实践。我们有三个目标：（i）研究AEX-HPLC方法的验证，包括通过AEX-HPLC和凝胶渗透-HPLC（GP-HPLC）测量的健康犬和高胆固醇血症犬脂蛋白组分中血清胆固醇浓度的相关性；（ii）建立通过AEX-HPLC测量的健康犬（n = 40）脂蛋白组分的参考区间；（iii）比较健康犬（n = 12）和高胆固醇血症犬（n = 23）血清中的脂蛋白组分。AEX-HPLC的分析重现性和精密度是可接受的。在健康犬和高胆固醇血症犬中，AEX-HPLC和GP-HPLC的总胆固醇（Total-Chol）、HDL胆固醇（HDL-Chol）、LDL胆固醇（LDL-Chol）+IDL胆固醇（IDL-Chol）以及VLDL胆固醇（VLDL-Chol）血清浓度之间存在正相关。通过AEX-HPLC测量的Total-Chol、HDL-Chol和LDL-Chol血清浓度的参考区间分别确定为2.97 - 9.32、2.79 - 6.57、0.16 - 3.28mmol/L（2.5 - 97.5%区间）。此外，健康犬和高胆固醇血症犬的脂蛋白谱存在显著差异。这些结果表明，AEX-HPLC可用于评估犬的脂蛋白谱，并且可能是犬高脂血症的一种新的有用指标。
