Mizutani Hisashi, Sako Toshinori, Arai Noriaki, Kuriyama Koji, Yoshimura Itaru, Mori Akihiro, Iwase Kenji, Hirose Hisashi
Veterinary Internal Medicine, Nippon Veterinary and Life Science University, Japan.
J Vet Med Sci. 2010 Jun;72(6):813-7. doi: 10.1292/jvms.09-0500. Epub 2010 Feb 9.
A high performance liquid chromatography system with a gel permeation column (GP-HPLC) and an on-line dual enzymatic system was applied to lipoprotein analysis in dogs. A high density lipoprotein (HDL) fraction obtained by conventional ultracentrifugation gave a single peak at around 28-29 min. Similarly, a low density lipoprotein (LDL) fraction gave single peak at around 24-25 min. The lipoprotein profiles of healthy dogs were contained large HDL peaks and small LDL peaks, and VLDL and CM were only marginally detected. In diabetic dogs, concentrations of VLDL-triglyceride and VLDL-total-cholesterol were elevated significantly. The lipoprotein profile analysis by GP-HPLC method would be useful in explication of abnormality of lipid metabolism in dogs.
采用配备凝胶渗透柱的高效液相色谱系统（GP-HPLC）和在线双酶系统对犬脂蛋白进行分析。通过传统超速离心法获得的高密度脂蛋白（HDL）组分在约28 - 29分钟处出现单峰。同样，低密度脂蛋白（LDL）组分在约24 - 25分钟处出现单峰。健康犬的脂蛋白谱包含大的HDL峰和小的LDL峰，极低密度脂蛋白（VLDL）和乳糜微粒（CM）仅微量检测到。在糖尿病犬中，VLDL-甘油三酯和VLDL-总胆固醇浓度显著升高。GP-HPLC法进行脂蛋白谱分析将有助于阐明犬脂质代谢异常情况。
